Advances in methods for identification and characterization of plant transporter function

TY - JOUR

T1 - Advances in methods for identification and characterization of plant transporter function

AU - Larsen, Bo

AU - Xu, Deyang

AU - Halkier, Barbara Ann

AU - Nour-Eldin, Hussam Hassan

PY - 2017/7/10

Y1 - 2017/7/10

N2 - Transport proteins are crucial for cellular function at all levels. Numerous importers and exporters facilitate transport of a diverse array of metabolites and ions intra- and intercellularly. Identification of transporter function is essential for understanding biological processes at both the cellular and organismal level. Assignment of a functional role to individual transporter proteins or to identify a transporter with a given substrate specificity has notoriously been challenging. Recently, major advances have been achieved in function-driven screens, phenotype-driven screens, and in silico-based approaches. In this review, we highlight examples that illustrate how new technology and tools have advanced identification and characterization of plant transporter functions.

AB - Transport proteins are crucial for cellular function at all levels. Numerous importers and exporters facilitate transport of a diverse array of metabolites and ions intra- and intercellularly. Identification of transporter function is essential for understanding biological processes at both the cellular and organismal level. Assignment of a functional role to individual transporter proteins or to identify a transporter with a given substrate specificity has notoriously been challenging. Recently, major advances have been achieved in function-driven screens, phenotype-driven screens, and in silico-based approaches. In this review, we highlight examples that illustrate how new technology and tools have advanced identification and characterization of plant transporter functions.

U2 - 10.1093/jxb/erx140

DO - 10.1093/jxb/erx140

M3 - Review

C2 - 28472492

SN - 0022-0957

VL - 68

SP - 4045

EP - 4056

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

IS - 15

ER -