Advances in characterizing ubiquitylation sites by mass spectrometry

TY - JOUR

T1 - Advances in characterizing ubiquitylation sites by mass spectrometry

AU - Sylvestersen, K.B.

AU - Young, C.

AU - Nielsen, M.L.

PY - 2013/2

Y1 - 2013/2

N2 - The attachment of one or more ubiquitin moieties to proteins plays a central regulatory mechanism in eukaryotic cells. Protein ubiquitylation regulates numerous cellular processes, including protein degradation, signal transduction, DNA repair and cell division. The characterization of ubiquitylation is a two-fold challenge that involves the mapping of ubiquitylation sites and the determination of ubiquitin chain topology. This review focuses on the technical advances in the mass spectrometry-based characterization of ubiquitylation sites, which have recently involved the large-scale identification of ubiquitylation sites by peptide-level enrichment strategies. The discovery that ubiquitylation is a widespread modification similar to phosphorylation and acetylation suggests cross-talk may also occur at the post translational modification level.

AB - The attachment of one or more ubiquitin moieties to proteins plays a central regulatory mechanism in eukaryotic cells. Protein ubiquitylation regulates numerous cellular processes, including protein degradation, signal transduction, DNA repair and cell division. The characterization of ubiquitylation is a two-fold challenge that involves the mapping of ubiquitylation sites and the determination of ubiquitin chain topology. This review focuses on the technical advances in the mass spectrometry-based characterization of ubiquitylation sites, which have recently involved the large-scale identification of ubiquitylation sites by peptide-level enrichment strategies. The discovery that ubiquitylation is a widespread modification similar to phosphorylation and acetylation suggests cross-talk may also occur at the post translational modification level.

UR - http://www.scopus.com/inward/record.url?scp=84875252687&partnerID=8YFLogxK

U2 - 10.1016/j.cbpa.2012.12.009

DO - 10.1016/j.cbpa.2012.12.009

M3 - Journal article

AN - SCOPUS:84875252687

SN - 1367-5931

VL - 17

SP - 49

EP - 58

JO - Current Opinion in Chemical Biology

JF - Current Opinion in Chemical Biology

IS - 1

ER -