Advanced microscopy of microbial cells

TY - JOUR

T1 - Advanced microscopy of microbial cells

AU - Haagensen, Janus Anders Juul

AU - Regenberg, Birgitte

AU - Sternberg, Claus

PY - 2011

Y1 - 2011

N2 - Growing awareness of heterogeneity in cells of microbial populations has emphasized the importance of advanced microscopy for visualization and understanding of the molecular mechanisms underlying cell-to-cell variation. In this review, we highlight some of the recent advances in confocal microscopy, super-resolution optical microscopy (STED, SIM, PALM) as well as atomic force microscopy and Raman spectroscopy. Using examples of bistability in microbial populations as well as biofilm development and differentiation in bacterial and yeast consortia, we demonstrate the importance of microscopy for visualization of variation between cells in phenotypic traits such as gene expression.

AB - Growing awareness of heterogeneity in cells of microbial populations has emphasized the importance of advanced microscopy for visualization and understanding of the molecular mechanisms underlying cell-to-cell variation. In this review, we highlight some of the recent advances in confocal microscopy, super-resolution optical microscopy (STED, SIM, PALM) as well as atomic force microscopy and Raman spectroscopy. Using examples of bistability in microbial populations as well as biofilm development and differentiation in bacterial and yeast consortia, we demonstrate the importance of microscopy for visualization of variation between cells in phenotypic traits such as gene expression.

KW - Faculty of Science

KW - Advanced microscopy techniques

KW - Single-cell gene expression

KW - Bistability

KW - Biofilm development and differentiation

KW - Bacteria

KW - Yeast

U2 - 10.1007/10_2010_83

DO - 10.1007/10_2010_83

M3 - Journal article

C2 - 21082308

SN - 0724-6145

VL - 124

SP - 21

EP - 54

JO - Advances in Biochemical Engineering. Biotechnology

JF - Advances in Biochemical Engineering. Biotechnology

ER -