Active nitrogen fixing heterotrophic bacteria at and below the chemocline of the central Baltic Sea

TY - JOUR

T1 - Active nitrogen fixing heterotrophic bacteria at and below the chemocline of the central Baltic Sea

AU - Farnelid, Hanna

AU - Bentzon-Tilia, Mikkel

AU - Andersson, Anders F.

AU - Bertilsson, Stefan

AU - Jost, Günter

AU - Labrenz, Matthias

AU - Jürgens, Klaus

AU - Riemann, Lasse

PY - 2013/7

Y1 - 2013/7

N2 - The Baltic Sea receives large nitrogen inputs by diazotrophic (N 2 -fixing) heterocystous cyanobacteria but the significance of heterotrophic N 2 fixation has not been studied. Here, the diversity, abundance and transcription of the nifH fragment of the nitrogenase enzyme in two basins of the Baltic Sea proper was examined. N 2 fixation was measured at the surface (5 m) and in anoxic water (200 m). Vertical sampling profiles of >10 and <10 μm size fractions were collected in 2007, 2008 and 2011 at the Gotland Deep and in 2011 in the Bornholm Basin. Both of these stations are characterized by permanently anoxic bottom water. The 454-pyrosequencing nifH analysis revealed a diverse assemblage of nifH genes related to alpha-, beta- and gammaproteobacteria (nifH cluster I) and anaerobic bacteria (nifH cluster III) at and below the chemocline. Abundances of genes and transcripts of seven diazotrophic phylotypes were investigated using quantitative polymerase chain reaction revealing abundances of heterotrophic nifH phylotypes of up to 2.1 × 10 7 nifH copies l -1. Abundant nifH transcripts (up to 3.2 × 10 4 transcripts l -1) within nifH cluster III and co-occurring N 2 fixation (0.44±0.26 nmol l -1 day -1) in deep water suggests that heterotrophic diazotrophs are fixing N 2 in anoxic ammonium-rich waters. Our results reveal that N 2 fixation in the Baltic Sea is not limited to illuminated N-deplete surface waters and suggest that N 2 fixation could also be of importance in other suboxic regions of the world's oceans.

AB - The Baltic Sea receives large nitrogen inputs by diazotrophic (N 2 -fixing) heterocystous cyanobacteria but the significance of heterotrophic N 2 fixation has not been studied. Here, the diversity, abundance and transcription of the nifH fragment of the nitrogenase enzyme in two basins of the Baltic Sea proper was examined. N 2 fixation was measured at the surface (5 m) and in anoxic water (200 m). Vertical sampling profiles of >10 and <10 μm size fractions were collected in 2007, 2008 and 2011 at the Gotland Deep and in 2011 in the Bornholm Basin. Both of these stations are characterized by permanently anoxic bottom water. The 454-pyrosequencing nifH analysis revealed a diverse assemblage of nifH genes related to alpha-, beta- and gammaproteobacteria (nifH cluster I) and anaerobic bacteria (nifH cluster III) at and below the chemocline. Abundances of genes and transcripts of seven diazotrophic phylotypes were investigated using quantitative polymerase chain reaction revealing abundances of heterotrophic nifH phylotypes of up to 2.1 × 10 7 nifH copies l -1. Abundant nifH transcripts (up to 3.2 × 10 4 transcripts l -1) within nifH cluster III and co-occurring N 2 fixation (0.44±0.26 nmol l -1 day -1) in deep water suggests that heterotrophic diazotrophs are fixing N 2 in anoxic ammonium-rich waters. Our results reveal that N 2 fixation in the Baltic Sea is not limited to illuminated N-deplete surface waters and suggest that N 2 fixation could also be of importance in other suboxic regions of the world's oceans.

U2 - 10.1038/ismej.2013.26

DO - 10.1038/ismej.2013.26

M3 - Journal article

C2 - 23446833

SN - 1751-7362

VL - 7

SP - 1413

EP - 1423

JO - I S M E Journal

JF - I S M E Journal

ER -