Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana

TY - JOUR

T1 - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana

AU - van der Graaff, Eric

AU - Dulk-Ras, A D

AU - Hooykaas, P J

AU - Keller, Bente

PY - 2000/11

Y1 - 2000/11

N2 - In a screen for leaf developmental mutants we have isolated an activator T-DNA-tagged mutant that produces leaves without a petiole. In addition to that leafy petiole phenotype this lettuce (let) mutant shows aberrant inflorescence branching and silique shape. The LEAFY PETIOLE (LEP) gene is located close to the right border of the T-DNA insert linked with these dominant phenotypes and encodes a protein with a domain with similarity to the DNA binding domain of members of the AP2/EREBP family of transcription factors. Introduction of the activation-tagged LEP gene in wild-type plants conferred all the phenotypic aberrations mentioned above. The leafy petiole phenotype consists of a conversion of the proximal part of the leaf from petiole into leaf blade, which means that leaf development in let is disturbed along the proximodistal axis. Therefore, LEP is involved in either cell division activity in the marginal meristem or patterning along the proximodistal axis.

AB - In a screen for leaf developmental mutants we have isolated an activator T-DNA-tagged mutant that produces leaves without a petiole. In addition to that leafy petiole phenotype this lettuce (let) mutant shows aberrant inflorescence branching and silique shape. The LEAFY PETIOLE (LEP) gene is located close to the right border of the T-DNA insert linked with these dominant phenotypes and encodes a protein with a domain with similarity to the DNA binding domain of members of the AP2/EREBP family of transcription factors. Introduction of the activation-tagged LEP gene in wild-type plants conferred all the phenotypic aberrations mentioned above. The leafy petiole phenotype consists of a conversion of the proximal part of the leaf from petiole into leaf blade, which means that leaf development in let is disturbed along the proximodistal axis. Therefore, LEP is involved in either cell division activity in the marginal meristem or patterning along the proximodistal axis.

KW - Arabidopsis

KW - Arabidopsis Proteins

KW - Base Sequence

KW - Cell Division

KW - DNA Primers

KW - DNA-Binding Proteins

KW - Gene Expression Regulation, Developmental

KW - Gene Expression Regulation, Plant

KW - Genes, Plant

KW - Mutation

KW - Phenotype

KW - Plant Leaves

KW - Plant Proteins

KW - Transcription Factors

M3 - Journal article

C2 - 11044410

SN - 0950-1991

VL - 127

SP - 4971

EP - 4980

JO - Development

JF - Development

IS - 22

ER -