Activation of human T lymphocytes by Leishmania lipophosphoglycan

TY - JOUR

T1 - Activation of human T lymphocytes by Leishmania lipophosphoglycan

AU - Kemp, M

AU - Theander, T G

AU - Handman, E

AU - Hey, A S

AU - Kurtzhals, J A

AU - Hviid, L

AU - Sørensen, A L

AU - Were, J O

AU - Koech, D K

AU - Kharazmi, A

N1 - Keywords: Animals; Antigens, Protozoan; Antinematodal Agents; Glycosphingolipids; Humans; Immunophenotyping; Interferon Type II; Leishmania tropica; Leishmaniasis, Visceral; Lymphocyte Activation; Metalloendopeptidases; Protozoan Proteins; T-Lymphocytes

PY - 1991

Y1 - 1991

N2 - This study describes Leishmania antigen-induced activation of lymphocytes isolated from Kenyan donors, previously treated for visceral leishmaniasis, and from Danish and Kenyan controls. Peripheral blood mononuclear cells (PBMC) from cured Kala-Azar patients proliferated and produced Interferon-gamma in vitro in response to lipophosphoglycan (LPG) isolated from Leishmania major. The proliferative response was mainly due to activation of CD2-positive T cells. PBMC from controls did not respond to LPG, but to sonicates prepared from both L. major and L. donovani promastigotes. The surface glycoprotein GP 63 failed to activate PBMC from any of the donors tested. These results show that the individuals cured from visceral leishmaniasis had expanded T-cell clones recognizing LPG, conceivably as a result of Leishmania infection. The LPG preparation was without detectable protein contamination. Thus, the results suggest that human T lymphocytes can respond to glycolipid antigens.

AB - This study describes Leishmania antigen-induced activation of lymphocytes isolated from Kenyan donors, previously treated for visceral leishmaniasis, and from Danish and Kenyan controls. Peripheral blood mononuclear cells (PBMC) from cured Kala-Azar patients proliferated and produced Interferon-gamma in vitro in response to lipophosphoglycan (LPG) isolated from Leishmania major. The proliferative response was mainly due to activation of CD2-positive T cells. PBMC from controls did not respond to LPG, but to sonicates prepared from both L. major and L. donovani promastigotes. The surface glycoprotein GP 63 failed to activate PBMC from any of the donors tested. These results show that the individuals cured from visceral leishmaniasis had expanded T-cell clones recognizing LPG, conceivably as a result of Leishmania infection. The LPG preparation was without detectable protein contamination. Thus, the results suggest that human T lymphocytes can respond to glycolipid antigens.

M3 - Journal article

C2 - 1902000

SN - 0301-6323

VL - 33

SP - 219

EP - 224

JO - Scandinavian Journal of Immunology, Supplement

JF - Scandinavian Journal of Immunology, Supplement

IS - 2

ER -