A Wingless and Notch double-repression mechanism regulates G1-S transition in the Drosophila wing

TY - JOUR

T1 - A Wingless and Notch double-repression mechanism regulates G1-S transition in the Drosophila wing

AU - Herranz, Héctor

AU - Pérez, Lidia

AU - Martín, Francisco A

AU - Milán, Marco

PY - 2008/6/4

Y1 - 2008/6/4

N2 - The control of tissue growth and patterning is orchestrated in various multicellular tissues by the coordinated activity of the signalling molecules Wnt/Wingless (Wg) and Notch, and mutations in these pathways can cause cancer. The role of these molecules in the control of cell proliferation and the crosstalk between their corresponding pathways remain poorly understood. Crosstalk between Notch and Wg has been proposed to organize pattern and growth in the Drosophila wing primordium. Here we report that Wg and Notch act in a surprisingly linear pathway to control G1-S progression. We present evidence that these molecules exert their function by regulating the expression of the dmyc proto-oncogene and the bantam micro-RNA, which positively modulated the activity of the E2F transcription factor. Our results demonstrate that Notch acts in this cellular context as a repressor of cell-cycle progression and Wg has a permissive role in alleviating Notch-mediated repression of G1-S progression in wing cells.

AB - The control of tissue growth and patterning is orchestrated in various multicellular tissues by the coordinated activity of the signalling molecules Wnt/Wingless (Wg) and Notch, and mutations in these pathways can cause cancer. The role of these molecules in the control of cell proliferation and the crosstalk between their corresponding pathways remain poorly understood. Crosstalk between Notch and Wg has been proposed to organize pattern and growth in the Drosophila wing primordium. Here we report that Wg and Notch act in a surprisingly linear pathway to control G1-S progression. We present evidence that these molecules exert their function by regulating the expression of the dmyc proto-oncogene and the bantam micro-RNA, which positively modulated the activity of the E2F transcription factor. Our results demonstrate that Notch acts in this cellular context as a repressor of cell-cycle progression and Wg has a permissive role in alleviating Notch-mediated repression of G1-S progression in wing cells.

KW - Animals

KW - DNA-Binding Proteins

KW - Drosophila Proteins

KW - Drosophila melanogaster

KW - G1 Phase

KW - MicroRNAs

KW - Proto-Oncogene Proteins

KW - Receptors, Notch

KW - Repressor Proteins

KW - S Phase

KW - Transcription Factors

KW - Wing

KW - Wnt1 Protein

U2 - 10.1038/emboj.2008.84

DO - 10.1038/emboj.2008.84

M3 - Journal article

C2 - 18451803

SN - 0261-4189

VL - 27

SP - 1633

EP - 1645

JO - E M B O Journal

JF - E M B O Journal

IS - 11

ER -