TY - CHAP
T1 - A western blot protocol for detection of proteins heterologously expressed in Xenopus laevis oocytes
AU - Jørgensen, Morten Egevang
AU - Nour-Eldin, Hussam Hassan
AU - Halkier, Barbara Ann
PY - 2016
Y1 - 2016
N2 - Oocytes of the African clawed frog, Xenopus laevis, are often used for expression and biochemical characterization of transporter proteins as the oocytes are particularly suitable for uptake assays and electrophysiological recordings. Assessment of the expression level of expressed transporters at the individual oocyte level is often desirable when comparing properties of wild type and mutant transporters. However, a large content of yolk platelets in the oocyte cytoplasm makes this a challenging task. Here we report a method for fast and easy, semiquantitative Western blot analysis of proteins heterologously expressed in Xenopus oocytes.
AB - Oocytes of the African clawed frog, Xenopus laevis, are often used for expression and biochemical characterization of transporter proteins as the oocytes are particularly suitable for uptake assays and electrophysiological recordings. Assessment of the expression level of expressed transporters at the individual oocyte level is often desirable when comparing properties of wild type and mutant transporters. However, a large content of yolk platelets in the oocyte cytoplasm makes this a challenging task. Here we report a method for fast and easy, semiquantitative Western blot analysis of proteins heterologously expressed in Xenopus oocytes.
U2 - 10.1007/978-1-4939-3393-8_10
DO - 10.1007/978-1-4939-3393-8_10
M3 - Book chapter
C2 - 26843169
SN - 978-1-4939-3391-4
T3 - Methods in Molecular Biology
SP - 99
EP - 107
BT - Biotechnology of plant secondary metabolism
A2 - Fett-Neto, Arthur Germano
PB - Springer
ER -