A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum

TY - JOUR

T1 - A versatile, high through-put, bead-based phagocytosis assay for Plasmodium falciparum

AU - Lloyd, Yukie M.

AU - Ngati, Elise P.

AU - Salanti, Ali

AU - Leke, Rose G.F.

AU - Taylor, Diane W.

PY - 2017

Y1 - 2017

N2 - Antibody-mediated phagocytosis is an important immune effector mechanism against Plasmodium falciparum-infected erythrocytes (IE); however, current phagocytosis assays use IE collected from infected individuals or from in vitro cultures of P. falciparum, making them prone to high variation. A simple, high-throughput flow cytometric assay was developed that uses THP-1 cells and fluorescent beads covalently-coupled with the malarial antigen VAR2CSA. The assay is highly repeatable, provides both the overall percent phagocytosis and semi-quantitates the number of antigen-coupled beads internalized.

AB - Antibody-mediated phagocytosis is an important immune effector mechanism against Plasmodium falciparum-infected erythrocytes (IE); however, current phagocytosis assays use IE collected from infected individuals or from in vitro cultures of P. falciparum, making them prone to high variation. A simple, high-throughput flow cytometric assay was developed that uses THP-1 cells and fluorescent beads covalently-coupled with the malarial antigen VAR2CSA. The assay is highly repeatable, provides both the overall percent phagocytosis and semi-quantitates the number of antigen-coupled beads internalized.

U2 - 10.1038/s41598-017-13900-4

DO - 10.1038/s41598-017-13900-4

M3 - Journal article

C2 - 29089635

AN - SCOPUS:85032685615

SN - 2045-2322

VL - 7

JO - Scientific Reports

JF - Scientific Reports

M1 - 14705

ER -