A TIMP-1 splice variant transcript: possible role in regulation of TIMP-1 expression

Nina Friesgård Øbro; Ulrik Axel Lademann; Karin Birkenkamp-Demtroder; Lars Holten-Andersen; Nils Brunner; Hanne Kjær Offenberg

doi:10.1016/j.canlet.2007.11.030

A TIMP-1 splice variant transcript: possible role in regulation of TIMP-1 expression

Nina Friesgård Øbro, Ulrik Axel Lademann, Karin Birkenkamp-Demtroder, Lars Holten-Andersen, Nils Brunner, Hanne Kjær Offenberg

3 Citationer (Scopus)

Abstract

A splice variant of tissue inhibitor of metalloproteinases-1 (TIMP-1) mRNA lacking exon 2 (TIMP-1-v2) has been identified in human cancer cells and in colorectal and breast cancer tumors. The purpose of this study was (1) to study the level of full length TIMP-1 and TIMP-1-v2 transcripts in colorectal tumors; (2) to investigate if TIMP-1-v2 is translated to protein. Full length TIMP-1 and TIMP-1-v2 mRNA levels were compared between colorectal tumors and normal mucosa by Q-PCR. Both full length TIMP-1 and TIMP-1-v2 transcripts were upregulated in tumor tissue. However, the level of TIMP-1-v2 relative to full length TIMP-1 was higher in normal compared to tumor tissue. Translation of TIMP-1-v2 to protein was analyzed in CHO cells. In this system, no TIMP-1-v2 protein was produced. Thus, the variant transcript seems to be an untranslated mRNA. These findings suggest that alternative splicing of TIMP-1 pre-mRNA to TIMP-1-v2 mRNA might be involved in regulating TIMP-1 expression.

Originalsprog	Engelsk
Tidsskrift	Cancer Letters
Vol/bind	262
Udgave nummer	1
Sider (fra-til)	64-70
Antal sider	7
ISSN	0304-3835
DOI	https://doi.org/10.1016/j.canlet.2007.11.030
Status	Udgivet - 2008

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10.1016/j.canlet.2007.11.030

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title = "A TIMP-1 splice variant transcript: possible role in regulation of TIMP-1 expression",

abstract = "A splice variant of tissue inhibitor of metalloproteinases-1 (TIMP-1) mRNA lacking exon 2 (TIMP-1-v2) has been identified in human cancer cells and in colorectal and breast cancer tumors. The purpose of this study was (1) to study the level of full length TIMP-1 and TIMP-1-v2 transcripts in colorectal tumors; (2) to investigate if TIMP-1-v2 is translated to protein. Full length TIMP-1 and TIMP-1-v2 mRNA levels were compared between colorectal tumors and normal mucosa by Q-PCR. Both full length TIMP-1 and TIMP-1-v2 transcripts were upregulated in tumor tissue. However, the level of TIMP-1-v2 relative to full length TIMP-1 was higher in normal compared to tumor tissue. Translation of TIMP-1-v2 to protein was analyzed in CHO cells. In this system, no TIMP-1-v2 protein was produced. Thus, the variant transcript seems to be an untranslated mRNA. These findings suggest that alternative splicing of TIMP-1 pre-mRNA to TIMP-1-v2 mRNA might be involved in regulating TIMP-1 expression.",

author = "{\O}bro, {Nina Friesg{\aa}rd} and Lademann, {Ulrik Axel} and Karin Birkenkamp-Demtroder and Lars Holten-Andersen and Nils Brunner and Offenberg, {Hanne Kj{\ae}r}",

year = "2008",

doi = "10.1016/j.canlet.2007.11.030",

language = "English",

volume = "262",

pages = "64--70",

journal = "Cancer Letters",

issn = "0304-3835",

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TY - JOUR

T1 - A TIMP-1 splice variant transcript

T2 - possible role in regulation of TIMP-1 expression

AU - Øbro, Nina Friesgård

AU - Lademann, Ulrik Axel

AU - Birkenkamp-Demtroder, Karin

AU - Holten-Andersen, Lars

AU - Brunner, Nils

AU - Offenberg, Hanne Kjær

PY - 2008

Y1 - 2008

N2 - A splice variant of tissue inhibitor of metalloproteinases-1 (TIMP-1) mRNA lacking exon 2 (TIMP-1-v2) has been identified in human cancer cells and in colorectal and breast cancer tumors. The purpose of this study was (1) to study the level of full length TIMP-1 and TIMP-1-v2 transcripts in colorectal tumors; (2) to investigate if TIMP-1-v2 is translated to protein. Full length TIMP-1 and TIMP-1-v2 mRNA levels were compared between colorectal tumors and normal mucosa by Q-PCR. Both full length TIMP-1 and TIMP-1-v2 transcripts were upregulated in tumor tissue. However, the level of TIMP-1-v2 relative to full length TIMP-1 was higher in normal compared to tumor tissue. Translation of TIMP-1-v2 to protein was analyzed in CHO cells. In this system, no TIMP-1-v2 protein was produced. Thus, the variant transcript seems to be an untranslated mRNA. These findings suggest that alternative splicing of TIMP-1 pre-mRNA to TIMP-1-v2 mRNA might be involved in regulating TIMP-1 expression.

AB - A splice variant of tissue inhibitor of metalloproteinases-1 (TIMP-1) mRNA lacking exon 2 (TIMP-1-v2) has been identified in human cancer cells and in colorectal and breast cancer tumors. The purpose of this study was (1) to study the level of full length TIMP-1 and TIMP-1-v2 transcripts in colorectal tumors; (2) to investigate if TIMP-1-v2 is translated to protein. Full length TIMP-1 and TIMP-1-v2 mRNA levels were compared between colorectal tumors and normal mucosa by Q-PCR. Both full length TIMP-1 and TIMP-1-v2 transcripts were upregulated in tumor tissue. However, the level of TIMP-1-v2 relative to full length TIMP-1 was higher in normal compared to tumor tissue. Translation of TIMP-1-v2 to protein was analyzed in CHO cells. In this system, no TIMP-1-v2 protein was produced. Thus, the variant transcript seems to be an untranslated mRNA. These findings suggest that alternative splicing of TIMP-1 pre-mRNA to TIMP-1-v2 mRNA might be involved in regulating TIMP-1 expression.

U2 - 10.1016/j.canlet.2007.11.030

DO - 10.1016/j.canlet.2007.11.030

M3 - Journal article

C2 - 18180096

SN - 0304-3835

VL - 262

SP - 64

EP - 70

JO - Cancer Letters

JF - Cancer Letters

IS - 1

ER -

A TIMP-1 splice variant transcript: possible role in regulation of TIMP-1 expression

Abstract

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