A snapshot of the physical and functional wiring of the Eps15 homology domain network in the nematode

Hanako Tsushima; Maria Grazia Malabarba; Stefano Confalonieri; Francesca Senic-Matuglia; Lisette G G C Verhoef; Cristina Bartocci; Giovanni D'Ario; Andrea Cocito; Pier Paolo Di Fiore; Anna Elisabetta Salcini

doi:10.1371/journal.pone.0056383

A snapshot of the physical and functional wiring of the Eps15 homology domain network in the nematode

Hanako Tsushima, Maria Grazia Malabarba, Stefano Confalonieri, Francesca Senic-Matuglia, Lisette G G C Verhoef, Cristina Bartocci, Giovanni D'Ario, Andrea Cocito, Pier Paolo Di Fiore, Anna Elisabetta Salcini

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Abstract

Protein interaction modules coordinate the connections within and the activity of intracellular signaling networks. The Eps15 Homology (EH) module, a protein-protein interaction domain that is a key feature of the EH-network, was originally identified in a few proteins involved in endocytosis and vesicle trafficking, and has subsequently also been implicated in actin reorganization, nuclear shuttling, and DNA repair. Here we report an extensive characterization of the physical connections and of the functional wirings of the EH-network in the nematode. Our data show that one of the major physiological roles of the EH-network is in neurotransmission. In addition, we found that the proteins of the network intersect, and possibly coordinate, a number of "territories" of cellular activity including endocytosis/recycling/vesicle transport, actin dynamics, general metabolism and signal transduction, ubiquitination/degradation of proteins, DNA replication/repair, and miRNA biogenesis and processing.

Originalsprog	Engelsk
Tidsskrift	PloS one
Vol/bind	8
Udgave nummer	2
Sider (fra-til)	e56383
ISSN	1932-6203
DOI	https://doi.org/10.1371/journal.pone.0056383
Status	Udgivet - 12 feb. 2013

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10.1371/journal.pone.0056383

PLoS One 2013-8(2)-e56383. doi 10.1371Forlagets udgivne version, 767 KB

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0056383

Citationsformater

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AU - Malabarba, Maria Grazia

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AU - Senic-Matuglia, Francesca

AU - Verhoef, Lisette G G C

AU - Bartocci, Cristina

AU - D'Ario, Giovanni

AU - Cocito, Andrea

AU - Di Fiore, Pier Paolo

AU - Salcini, Anna Elisabetta

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AB - Protein interaction modules coordinate the connections within and the activity of intracellular signaling networks. The Eps15 Homology (EH) module, a protein-protein interaction domain that is a key feature of the EH-network, was originally identified in a few proteins involved in endocytosis and vesicle trafficking, and has subsequently also been implicated in actin reorganization, nuclear shuttling, and DNA repair. Here we report an extensive characterization of the physical connections and of the functional wirings of the EH-network in the nematode. Our data show that one of the major physiological roles of the EH-network is in neurotransmission. In addition, we found that the proteins of the network intersect, and possibly coordinate, a number of "territories" of cellular activity including endocytosis/recycling/vesicle transport, actin dynamics, general metabolism and signal transduction, ubiquitination/degradation of proteins, DNA replication/repair, and miRNA biogenesis and processing.

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KW - Reproducibility of Results

KW - Synaptic Transmission

KW - Two-Hybrid System Techniques

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A snapshot of the physical and functional wiring of the Eps15 homology domain network in the nematode

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