A snapshot of the physical and functional wiring of the Eps15 homology domain network in the nematode

Hanako Tsushima; Maria Grazia Malabarba; Stefano Confalonieri; Francesca Senic-Matuglia; Lisette G G C Verhoef; Cristina Bartocci; Giovanni D'Ario; Andrea Cocito; Pier Paolo Di Fiore; Anna Elisabetta Salcini

doi:10.1371/journal.pone.0056383

A snapshot of the physical and functional wiring of the Eps15 homology domain network in the nematode

Hanako Tsushima, Maria Grazia Malabarba, Stefano Confalonieri, Francesca Senic-Matuglia, Lisette G G C Verhoef, Cristina Bartocci, Giovanni D'Ario, Andrea Cocito, Pier Paolo Di Fiore, Anna Elisabetta Salcini

1 Citationer (Scopus)

1144 Downloads (Pure)

Abstract

Protein interaction modules coordinate the connections within and the activity of intracellular signaling networks. The Eps15 Homology (EH) module, a protein-protein interaction domain that is a key feature of the EH-network, was originally identified in a few proteins involved in endocytosis and vesicle trafficking, and has subsequently also been implicated in actin reorganization, nuclear shuttling, and DNA repair. Here we report an extensive characterization of the physical connections and of the functional wirings of the EH-network in the nematode. Our data show that one of the major physiological roles of the EH-network is in neurotransmission. In addition, we found that the proteins of the network intersect, and possibly coordinate, a number of "territories" of cellular activity including endocytosis/recycling/vesicle transport, actin dynamics, general metabolism and signal transduction, ubiquitination/degradation of proteins, DNA replication/repair, and miRNA biogenesis and processing.

Originalsprog	Engelsk
Tidsskrift	PloS one
Vol/bind	8
Udgave nummer	2
Sider (fra-til)	e56383
ISSN	1932-6203
DOI	https://doi.org/10.1371/journal.pone.0056383
Status	Udgivet - 12 feb. 2013

Adgang til dokumentet

10.1371/journal.pone.0056383

PLoS One 2013-8(2)-e56383. doi 10.1371Forlagets udgivne version, 767 KB

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0056383

Citationsformater

@article{42b8ba56615e441d850505fa0d76194f,

title = "A snapshot of the physical and functional wiring of the Eps15 homology domain network in the nematode",

abstract = "Protein interaction modules coordinate the connections within and the activity of intracellular signaling networks. The Eps15 Homology (EH) module, a protein-protein interaction domain that is a key feature of the EH-network, was originally identified in a few proteins involved in endocytosis and vesicle trafficking, and has subsequently also been implicated in actin reorganization, nuclear shuttling, and DNA repair. Here we report an extensive characterization of the physical connections and of the functional wirings of the EH-network in the nematode. Our data show that one of the major physiological roles of the EH-network is in neurotransmission. In addition, we found that the proteins of the network intersect, and possibly coordinate, a number of {"}territories{"} of cellular activity including endocytosis/recycling/vesicle transport, actin dynamics, general metabolism and signal transduction, ubiquitination/degradation of proteins, DNA replication/repair, and miRNA biogenesis and processing.",

keywords = "Adaptor Proteins, Vesicular Transport, Animals, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Gene Expression Regulation, Protein Structure, Tertiary, Reproducibility of Results, Synaptic Transmission, Two-Hybrid System Techniques",

author = "Hanako Tsushima and Malabarba, {Maria Grazia} and Stefano Confalonieri and Francesca Senic-Matuglia and Verhoef, {Lisette G G C} and Cristina Bartocci and Giovanni D'Ario and Andrea Cocito and {Di Fiore}, {Pier Paolo} and Salcini, {Anna Elisabetta}",

year = "2013",

month = feb,

day = "12",

doi = "10.1371/journal.pone.0056383",

language = "English",

volume = "8",

pages = "e56383",

journal = "PLoS Computational Biology",

issn = "1932-6203",

publisher = "Public Library of Science",

number = "2",

}

TY - JOUR

T1 - A snapshot of the physical and functional wiring of the Eps15 homology domain network in the nematode

AU - Tsushima, Hanako

AU - Malabarba, Maria Grazia

AU - Confalonieri, Stefano

AU - Senic-Matuglia, Francesca

AU - Verhoef, Lisette G G C

AU - Bartocci, Cristina

AU - D'Ario, Giovanni

AU - Cocito, Andrea

AU - Di Fiore, Pier Paolo

AU - Salcini, Anna Elisabetta

PY - 2013/2/12

Y1 - 2013/2/12

N2 - Protein interaction modules coordinate the connections within and the activity of intracellular signaling networks. The Eps15 Homology (EH) module, a protein-protein interaction domain that is a key feature of the EH-network, was originally identified in a few proteins involved in endocytosis and vesicle trafficking, and has subsequently also been implicated in actin reorganization, nuclear shuttling, and DNA repair. Here we report an extensive characterization of the physical connections and of the functional wirings of the EH-network in the nematode. Our data show that one of the major physiological roles of the EH-network is in neurotransmission. In addition, we found that the proteins of the network intersect, and possibly coordinate, a number of "territories" of cellular activity including endocytosis/recycling/vesicle transport, actin dynamics, general metabolism and signal transduction, ubiquitination/degradation of proteins, DNA replication/repair, and miRNA biogenesis and processing.

AB - Protein interaction modules coordinate the connections within and the activity of intracellular signaling networks. The Eps15 Homology (EH) module, a protein-protein interaction domain that is a key feature of the EH-network, was originally identified in a few proteins involved in endocytosis and vesicle trafficking, and has subsequently also been implicated in actin reorganization, nuclear shuttling, and DNA repair. Here we report an extensive characterization of the physical connections and of the functional wirings of the EH-network in the nematode. Our data show that one of the major physiological roles of the EH-network is in neurotransmission. In addition, we found that the proteins of the network intersect, and possibly coordinate, a number of "territories" of cellular activity including endocytosis/recycling/vesicle transport, actin dynamics, general metabolism and signal transduction, ubiquitination/degradation of proteins, DNA replication/repair, and miRNA biogenesis and processing.

KW - Adaptor Proteins, Vesicular Transport

KW - Animals

KW - Caenorhabditis elegans

KW - Caenorhabditis elegans Proteins

KW - Gene Expression Regulation

KW - Protein Structure, Tertiary

KW - Reproducibility of Results

KW - Synaptic Transmission

KW - Two-Hybrid System Techniques

U2 - 10.1371/journal.pone.0056383

DO - 10.1371/journal.pone.0056383

M3 - Journal article

C2 - 23424658

SN - 1932-6203

VL - 8

SP - e56383

JO - PLoS Computational Biology

JF - PLoS Computational Biology

IS - 2

ER -

A snapshot of the physical and functional wiring of the Eps15 homology domain network in the nematode

Abstract

Adgang til dokumentet

Fingeraftryk

Citationsformater