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A rapid radiochemical filter paper assay for determination of hexokinase activity and affinity for glucose-6-phosphate
Janne Rasmuss Hingst, Rie Dybboe Bjerre,
Jørgen Wojtaszewski
, Jørgen Jensen
*
*
Corresponding author af dette arbejde
August Krogh Sektionen for Molekylær Fysiologi
1
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Keyphrases
Glucose-6-phosphatase (G6Pase)
100%
Hexokinase
100%
2-Deoxy-d-glucose (2-DG)
28%
Feedback Inhibition
14%
Glucose Phosphorylation
14%
Natural Products
7%
Phosphate
7%
Maximal Velocity
7%
Time Dependency
7%
Physiological Condition
7%
Nicotinamide Adenine Dinucleotide Phosphate (NADPH)
7%
Linear Time
7%
Linear Concentration
7%
Enzyme Activity
7%
Product Formation
7%
Simple Assay
7%
Physiological Regulation
7%
Mitochondria
7%
Concentration Dependency
7%
Glucose Metabolism
7%
Hexose
7%
Rate-limiting Step
7%
Gluconolactone
7%
Biochemistry, Genetics and Molecular Biology
Hexokinase
100%
Glucose 6-Phosphate
100%
2-Deoxy-D-Glucose
28%
Product Inhibition
14%
Hexose
7%
Glucose Metabolism
7%
Concentration Response
7%
Enzyme Activity
7%
Mitochondrion
7%