A Rapid Bacteriophage DNA Extraction Method

Dziuginta Jakociune; Arshnee Moodley

doi:10.3390/mps1030027

A Rapid Bacteriophage DNA Extraction Method

Dziuginta Jakociune, Arshnee Moodley

6 Citationer (Scopus)

54 Downloads (Pure)

Abstract

Bacteriophages (phages) are intensely investigated as non-antibiotic alternatives to circumvent antibiotic resistance development as well as last resort therapeutic options against antibiotic resistant bacteria. As part of gaining a better understanding of phages and to determine if phages harbor putative virulence factors, whole genome sequencing is used, for which good quality phage DNA is needed. Traditional phage DNA extraction methods are tedious and time consuming, requiring specialized equipment e.g., an ultra-centrifuge. Here, we describe a quick and simple method (under four hours) to extract DNA from double stranded DNA (dsDNA) phages at titers above 1.0 × 1010 plaque-forming units (PFU)/mL. This DNA was suitable for library preparation using the Nextera XT kit and sequencing on the Illumina MiSeq platform

Originalsprog	Engelsk
Artikelnummer	27
Tidsskrift	Biology Methods and Protocols
Vol/bind	1
Udgave nummer	3
ISSN	2396-8923
DOI	https://doi.org/10.3390/mps1030027
Status	Udgivet - 2018

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10.3390/mps1030027Licens: CC BY

A Rapid Bacteriophage DNA Extraction MethodForlagets udgivne version, 563 KBLicens: CC BY

Citationsformater

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abstract = "Bacteriophages (phages) are intensely investigated as non-antibiotic alternatives to circumvent antibiotic resistance development as well as last resort therapeutic options against antibiotic resistant bacteria. As part of gaining a better understanding of phages and to determine if phages harbor putative virulence factors, whole genome sequencing is used, for which good quality phage DNA is needed. Traditional phage DNA extraction methods are tedious and time consuming, requiring specialized equipment e.g., an ultra-centrifuge. Here, we describe a quick and simple method (under four hours) to extract DNA from double stranded DNA (dsDNA) phages at titers above 1.0 × 1010 plaque-forming units (PFU)/mL. This DNA was suitable for library preparation using the Nextera XT kit and sequencing on the Illumina MiSeq platform",

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AU - Jakociune, Dziuginta

AU - Moodley, Arshnee

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AB - Bacteriophages (phages) are intensely investigated as non-antibiotic alternatives to circumvent antibiotic resistance development as well as last resort therapeutic options against antibiotic resistant bacteria. As part of gaining a better understanding of phages and to determine if phages harbor putative virulence factors, whole genome sequencing is used, for which good quality phage DNA is needed. Traditional phage DNA extraction methods are tedious and time consuming, requiring specialized equipment e.g., an ultra-centrifuge. Here, we describe a quick and simple method (under four hours) to extract DNA from double stranded DNA (dsDNA) phages at titers above 1.0 × 1010 plaque-forming units (PFU)/mL. This DNA was suitable for library preparation using the Nextera XT kit and sequencing on the Illumina MiSeq platform

U2 - 10.3390/mps1030027

DO - 10.3390/mps1030027

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JO - Biology Methods and Protocols

JF - Biology Methods and Protocols

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ER -

A Rapid Bacteriophage DNA Extraction Method

Abstract

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