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Abstract
A PORCINE MODEL OF HAEMATOGENOUS BRAIN INFECTION WITH STAPHYLOCOCCUS AUREUS
Astrup Lærke1, Agerholm Jørgen1, Nielsen Ole1, Jensen Henrik1,
Leifsson Páll1, Iburg Tine2.
1: Faculty of Health and Medical Sciences, University of Copenhagen, Denmark
[email protected]
2: National Veterinary Institute, Uppsala, Sweden
Introduction
Staphylococcus aureus (S.aureus) is a common cause of sepsis and brain abscesses in man and a frequent cause of porcine pyaemia. Here we present a porcine model of haematogenous S. aureus-induced brain infection.
Materials and Methods
Four pigs had two intravenous catheters inserted surgically, one in a. carotis communis and one in v. jugularis externa. All pigs received 106 CFU/kg body weight S. aureus through the arterial catheter. Bacteria were either suspended in isotonic saline infused at constant flow for 60 minutes (two pigs) or given as a bolus injection of autologoue thromboemboli (two pigs). The venous catheter was used for blood sampling before, during and after inoculation. The pigs were euthanized either 24 or 48 hours after inoculation. The brains were collected and examined histologically.
Results
We describe unifocal suppurative encephalitis 48 hours after inoculation with autologoue S. aureus thromoemboli. The other pigs developed mild diffuse cerebral oedema. The blood analyses showed a short period of bacteraemia.
Conclusion
Our pilot study proves it possible to establish embolic infection in the porcine brain through the normal circulatory pathway, when autologue thromboemboli are used. This was hitherto thought impossible due to the presence of rete mirabilis in this species.
Astrup Lærke1, Agerholm Jørgen1, Nielsen Ole1, Jensen Henrik1,
Leifsson Páll1, Iburg Tine2.
1: Faculty of Health and Medical Sciences, University of Copenhagen, Denmark
[email protected]
2: National Veterinary Institute, Uppsala, Sweden
Introduction
Staphylococcus aureus (S.aureus) is a common cause of sepsis and brain abscesses in man and a frequent cause of porcine pyaemia. Here we present a porcine model of haematogenous S. aureus-induced brain infection.
Materials and Methods
Four pigs had two intravenous catheters inserted surgically, one in a. carotis communis and one in v. jugularis externa. All pigs received 106 CFU/kg body weight S. aureus through the arterial catheter. Bacteria were either suspended in isotonic saline infused at constant flow for 60 minutes (two pigs) or given as a bolus injection of autologoue thromboemboli (two pigs). The venous catheter was used for blood sampling before, during and after inoculation. The pigs were euthanized either 24 or 48 hours after inoculation. The brains were collected and examined histologically.
Results
We describe unifocal suppurative encephalitis 48 hours after inoculation with autologoue S. aureus thromoemboli. The other pigs developed mild diffuse cerebral oedema. The blood analyses showed a short period of bacteraemia.
Conclusion
Our pilot study proves it possible to establish embolic infection in the porcine brain through the normal circulatory pathway, when autologue thromboemboli are used. This was hitherto thought impossible due to the presence of rete mirabilis in this species.
| Bidragets oversatte titel | En porcin model af hæmatogen hjerneinfektion med staphylococcus aureus |
|---|---|
| Originalsprog | Engelsk |
| Publikationsdato | 2012 |
| Antal sider | 1 |
| Status | Udgivet - 2012 |
Emneord
- Det tidligere LIFE
- Animal models
- brain
- Staphylococcus aureus
Aktiviteter
- 1 Foredrag og mundtlige bidrag
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A porcine model of haematogenous brain infection with staphylococcus aureus
Lærke Boye Astrup (Foredragsholder)
7 sep. 2012Aktivitet: Tale eller præsentation - typer › Foredrag og mundtlige bidrag