TY - JOUR
T1 - A peptide-gated ion channel from the freshwater polyp Hydra.
AU - Golubovic, Andjelko
AU - Kuhn, Anne
AU - Williamson, Michael
AU - Kalbacher, Hubert
AU - Holstein, Thomas W
AU - Grimmelikhuijzen, Cornelis J P
AU - Gründer, Stefan
N1 - Keywords: Amino Acid Sequence; Animals; Cloning, Molecular; Hydra; In Situ Hybridization; Ions; Ligands; Membrane Proteins; Molecular Sequence Data; Nerve Tissue Proteins; Peptides; Phylogeny; Rats; Sequence Homology, Amino Acid; Sodium Channels
PY - 2007
Y1 - 2007
N2 - Chemical transmitters are either low molecular weight molecules or neuropeptides. As a general rule, neuropeptides activate only slow metabotropic receptors. To date, only one exception to this rule is known, the FMRFamide-activated Na(+) channel (FaNaC) from snails. Until now FaNaC has been regarded as a curiosity, and it was not known whether peptide-gated ionotropic receptors are also present in other animal groups. Nervous systems first evolved in cnidarians, which extensively use neuropeptides. Here we report cloning from the freshwater cnidarian Hydra of a novel ion channel (Hydra sodium channel, HyNaC) that is directly gated by the neuropeptides Hydra-RFamides I and II and is related to FaNaC. The cells expressing HyNaC localize to the base of the tentacles, adjacent to the neurons producing the Hydra-RFamides, suggesting that the peptides are the natural ligands for this channel. Our results suggest that neuropeptides were already used for fast transmission in ancient nervous systems.
AB - Chemical transmitters are either low molecular weight molecules or neuropeptides. As a general rule, neuropeptides activate only slow metabotropic receptors. To date, only one exception to this rule is known, the FMRFamide-activated Na(+) channel (FaNaC) from snails. Until now FaNaC has been regarded as a curiosity, and it was not known whether peptide-gated ionotropic receptors are also present in other animal groups. Nervous systems first evolved in cnidarians, which extensively use neuropeptides. Here we report cloning from the freshwater cnidarian Hydra of a novel ion channel (Hydra sodium channel, HyNaC) that is directly gated by the neuropeptides Hydra-RFamides I and II and is related to FaNaC. The cells expressing HyNaC localize to the base of the tentacles, adjacent to the neurons producing the Hydra-RFamides, suggesting that the peptides are the natural ligands for this channel. Our results suggest that neuropeptides were already used for fast transmission in ancient nervous systems.
U2 - 10.1074/jbc.M706849200
DO - 10.1074/jbc.M706849200
M3 - Journal article
C2 - 17911098
SN - 0021-9258
VL - 282
SP - 35098
EP - 35103
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 48
ER -