TY - JOUR
T1 - A novel Sulfolobus non-conjugative extrachromosomal genetic element capable of integration into the host genome and spreading in the presence of a fusellovirus
AU - Wang, Ying
AU - Duan, Zhenhong
AU - Zhu, Haojun
AU - Guo, Xin
AU - Wang, Ziyi
AU - Zhou, Ju
AU - She, Qunxin
AU - Huang, Li
N1 - Keywords: Amino Acid Sequence; Attachment Sites, Microbiological; Base Sequence; Catalytic Domain; Fuselloviridae; Gene Transfer, Horizontal; Integrases; Molecular Sequence Data; Open Reading Frames; Recombination, Genetic; Sequence Alignment; Sulfolobus solfataricus
PY - 2007
Y1 - 2007
N2 - An integrative non-conjugative extrachromosomal genetic element, denoted as pSSVi, has been isolated from a Sulfolobus solfataricus P2 strain and was characterized. This genetic element is a double-stranded DNA of 5740 bp in size and contains eight open reading frames (ORFs). It resembles members of the pRN plasmid family in genome organization but shows only weak similarity to the latter in conserved regions. pSSVi has a copG gene similar to that of a pRN plasmid, encodes a large replication protein which, unlike a typical pRN RepA, contains no polymerase/primase domain, and lacks the plrA gene. Interestingly, pSSVi encodes an SSV-type integrase which probably catalyzes the integration of its genome into a specific site (a tRNA(Arg) gene) in the S. solfataricus P2 genome. Like pSSVx, pSSVi can be packaged into a spindle-like viral particle and spread with the help of SSV1 or SSV2. In addition, both SSV1 and SSV2 appeared to replicate more efficiently in the presence of pSSVi. Given the versatile genetic abilities, pSSVi appears to be well suited for a role in horizontal gene transfer.
AB - An integrative non-conjugative extrachromosomal genetic element, denoted as pSSVi, has been isolated from a Sulfolobus solfataricus P2 strain and was characterized. This genetic element is a double-stranded DNA of 5740 bp in size and contains eight open reading frames (ORFs). It resembles members of the pRN plasmid family in genome organization but shows only weak similarity to the latter in conserved regions. pSSVi has a copG gene similar to that of a pRN plasmid, encodes a large replication protein which, unlike a typical pRN RepA, contains no polymerase/primase domain, and lacks the plrA gene. Interestingly, pSSVi encodes an SSV-type integrase which probably catalyzes the integration of its genome into a specific site (a tRNA(Arg) gene) in the S. solfataricus P2 genome. Like pSSVx, pSSVi can be packaged into a spindle-like viral particle and spread with the help of SSV1 or SSV2. In addition, both SSV1 and SSV2 appeared to replicate more efficiently in the presence of pSSVi. Given the versatile genetic abilities, pSSVi appears to be well suited for a role in horizontal gene transfer.
U2 - 10.1016/j.virol.2007.01.035
DO - 10.1016/j.virol.2007.01.035
M3 - Journal article
C2 - 17331555
SN - 0042-6822
VL - 363
SP - 124
EP - 133
JO - Virology
JF - Virology
IS - 1
ER -