@article{5aaefe6001c011deb05e000ea68e967b,
title = "A Novel Method for Detection of Glycoproteins on Sodium Dodecyl Sulphate Polyacrylamide Gel Using Radio-Iodinated Tyrosine.",
abstract = "The aim of this study is to develop a novel method for detection of glycoproteins on polyacrylamide gel. In this method, radio-iodinated-tyrosine (125I-tyrosine) was conjugated to glycoprotein by schiff's base mechanism on the sodium dodecyl sulfate- polyacrylamide gel. Ovalbumin and Concanavalin A (Con A) were used as a glycosylated and a non-glycosylated model proteins, respectively. The proteins were separated in SDS- PAGE and oligosaccharides on the glycoprotein were oxidised using periodic acid to produce aldehydes than 125I-tyroine was conjugated to aldehyde groups without using reducing agent like Sodium Metabisulfite. The radio-iodinated glycoprotein on gel was scanned using a Multi-Photon Detection (MPD) scanner. The elechtrophoretic analysis of ovalbumin and Con A were performed and stained with Coomassie brilliant blue to identify total proteins, while MPD detection of glycoproteins using 125I-tyrosine selectively detected ovalbumin. Present results showed that MPD enhanced glycoprotein detection method can be used as a sensitive tool for the detection of glycoproteins on polyacrylamide gel",
author = "Amarnadh Nalla and Draz, {Hossam M.} and Anita Dole and Hansen, {Thorkild C-B{\o}g} and Drukier, {Andrzej K.}",
year = "2009",
language = "English",
volume = "3",
pages = "18--24",
journal = "International Journal of Biological Chemistry",
issn = "1819-155X",
publisher = "Academic Journals Inc",
number = "1",
}