TY - JOUR
T1 - A non-traumatic Staphylococcus aureus osteomyelistis model in pigs
AU - Jensen, Henrik Michael Elvang
AU - Nielsen, Ole Lerberg
AU - Agerholm, Jørgen Steen
AU - Iburg, Tine Moesgaard
AU - Johansen, Louise Kruse
AU - Johannesson, Eleonora
AU - Møller, Maiken
AU - Jahn, Line
AU - Munk, Liselotte
AU - Aalbæk, Bent
AU - Leifsson, Pall Skuli
PY - 2010
Y1 - 2010
N2 - Background: The propensity for bacterial localization within bones of juvenile pigs is similar to the situation in humans, where haematogenously based osteomyelitis most commonly occurs in infants and children. In both pigs and humans. Staphylococcus aureus is a dominant cause of pyaemic lesions including osteomyelitis. The aim of the present study was to evaluate the pig as a model for the development of osteomyelitis following haematogenous spread of S. aureus. Materials and Methods: Twelve animals were challenged intravenously once or twice with 1×108 bacteria/kg body weight and euthanased consecutively from 6 h to 48 h after challenge. Following euthanasia, tissues were sampled from the lungs and bones for histology and immunohistochemical staining of vessels, different inflammatiory cells, apoptosis cells, and S. aureus. Results: Disseminated microabscesses developed within the lungs by 6 h but had disappeared at 48 h. Within the metaphyseal area of bones, microabscesses developed after 12 h and progressed until 48 h after challenge. Within bones, lesions were localized in separate foci from where the infection progressed towards the growth plate, which was in some cases bypassed due to bacterial spread through transphyseal vascular channels. Often, bone lesions resulted in trabecular osteosis, in which apoptotic cells were sometimes present. Conclusion: The model revealed a pattern of development and presence of lesions similar to the frequently occurring osteomyelitic lesions, especially in prepubertal children following haematogenous spread of S. aureus. Therefore, this model can be reliably applied in studies of this disease with respect to pathophysiology, pathomorphology, impact of strain virulence, and therapy.
AB - Background: The propensity for bacterial localization within bones of juvenile pigs is similar to the situation in humans, where haematogenously based osteomyelitis most commonly occurs in infants and children. In both pigs and humans. Staphylococcus aureus is a dominant cause of pyaemic lesions including osteomyelitis. The aim of the present study was to evaluate the pig as a model for the development of osteomyelitis following haematogenous spread of S. aureus. Materials and Methods: Twelve animals were challenged intravenously once or twice with 1×108 bacteria/kg body weight and euthanased consecutively from 6 h to 48 h after challenge. Following euthanasia, tissues were sampled from the lungs and bones for histology and immunohistochemical staining of vessels, different inflammatiory cells, apoptosis cells, and S. aureus. Results: Disseminated microabscesses developed within the lungs by 6 h but had disappeared at 48 h. Within the metaphyseal area of bones, microabscesses developed after 12 h and progressed until 48 h after challenge. Within bones, lesions were localized in separate foci from where the infection progressed towards the growth plate, which was in some cases bypassed due to bacterial spread through transphyseal vascular channels. Often, bone lesions resulted in trabecular osteosis, in which apoptotic cells were sometimes present. Conclusion: The model revealed a pattern of development and presence of lesions similar to the frequently occurring osteomyelitic lesions, especially in prepubertal children following haematogenous spread of S. aureus. Therefore, this model can be reliably applied in studies of this disease with respect to pathophysiology, pathomorphology, impact of strain virulence, and therapy.
M3 - Journal article
SN - 0258-851X
VL - 24
SP - 257
EP - 264
JO - In Vivo
JF - In Vivo
ER -