A monoclonal antibody for G protein-coupled receptor crystallography

Peter W Day; Søren Gøgsig Faarup Rasmussen; Charles Parnot; Juan José Fung; Asna Masood; Tong Sun Kobilka; Xiao-Jie Yao; Hee-Jung Choi; William I Weis; Daniel K Rohrer; Brian K Kobilka

doi:10.1038/nmeth1112

A monoclonal antibody for G protein-coupled receptor crystallography

Peter W Day, Søren Gøgsig Faarup Rasmussen, Charles Parnot, Juan José Fung, Asna Masood, Tong Sun Kobilka, Xiao-Jie Yao, Hee-Jung Choi, William I Weis, Daniel K Rohrer, Brian K Kobilka

Neuropharm and Genetics

103 Citationer (Scopus)

Abstract

G protein-coupled receptors (GPCRs) constitute the largest family of signaling proteins in mammals, mediating responses to hormones, neurotransmitters, and senses of sight, smell and taste. Mechanistic insight into GPCR signal transduction is limited by a paucity of high-resolution structural information. We describe the generation of a monoclonal antibody that recognizes the third intracellular loop (IL3) of the native human beta(2) adrenergic (beta(2)AR) receptor; this antibody was critical for acquiring diffraction-quality crystals.

Originalsprog	Engelsk
Tidsskrift	Nature Methods
Vol/bind	4
Udgave nummer	11
Sider (fra-til)	927-9
Antal sider	3
ISSN	1548-7091
DOI	https://doi.org/10.1038/nmeth1112
Status	Udgivet - nov. 2007

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10.1038/nmeth1112

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title = "A monoclonal antibody for G protein-coupled receptor crystallography",

abstract = "G protein-coupled receptors (GPCRs) constitute the largest family of signaling proteins in mammals, mediating responses to hormones, neurotransmitters, and senses of sight, smell and taste. Mechanistic insight into GPCR signal transduction is limited by a paucity of high-resolution structural information. We describe the generation of a monoclonal antibody that recognizes the third intracellular loop (IL3) of the native human beta(2) adrenergic (beta(2)AR) receptor; this antibody was critical for acquiring diffraction-quality crystals.",

keywords = "Amino Acid Sequence, Animals, Antibodies, Monoclonal, Antigen-Antibody Reactions, Blotting, Western, Crystallization, Crystallography, Epitopes, Fluorescent Dyes, Humans, Immunoglobulin Fab Fragments, Mice, Molecular Sequence Data, Protein Structure, Tertiary, Receptors, Adrenergic, beta-2, Receptors, G-Protein-Coupled, Recombinant Proteins, Rhodamines, Vaccination",

author = "Day, {Peter W} and Rasmussen, {S{\o}ren G{\o}gsig Faarup} and Charles Parnot and Fung, {Juan Jos{\'e}} and Asna Masood and Kobilka, {Tong Sun} and Xiao-Jie Yao and Hee-Jung Choi and Weis, {William I} and Rohrer, {Daniel K} and Kobilka, {Brian K}",

year = "2007",

month = nov,

doi = "10.1038/nmeth1112",

language = "English",

volume = "4",

pages = "927--9",

journal = "Nature Methods",

issn = "1548-7091",

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TY - JOUR

T1 - A monoclonal antibody for G protein-coupled receptor crystallography

AU - Day, Peter W

AU - Rasmussen, Søren Gøgsig Faarup

AU - Parnot, Charles

AU - Fung, Juan José

AU - Masood, Asna

AU - Kobilka, Tong Sun

AU - Yao, Xiao-Jie

AU - Choi, Hee-Jung

AU - Weis, William I

AU - Rohrer, Daniel K

AU - Kobilka, Brian K

PY - 2007/11

Y1 - 2007/11

N2 - G protein-coupled receptors (GPCRs) constitute the largest family of signaling proteins in mammals, mediating responses to hormones, neurotransmitters, and senses of sight, smell and taste. Mechanistic insight into GPCR signal transduction is limited by a paucity of high-resolution structural information. We describe the generation of a monoclonal antibody that recognizes the third intracellular loop (IL3) of the native human beta(2) adrenergic (beta(2)AR) receptor; this antibody was critical for acquiring diffraction-quality crystals.

AB - G protein-coupled receptors (GPCRs) constitute the largest family of signaling proteins in mammals, mediating responses to hormones, neurotransmitters, and senses of sight, smell and taste. Mechanistic insight into GPCR signal transduction is limited by a paucity of high-resolution structural information. We describe the generation of a monoclonal antibody that recognizes the third intracellular loop (IL3) of the native human beta(2) adrenergic (beta(2)AR) receptor; this antibody was critical for acquiring diffraction-quality crystals.

KW - Amino Acid Sequence

KW - Animals

KW - Antibodies, Monoclonal

KW - Antigen-Antibody Reactions

KW - Blotting, Western

KW - Crystallization

KW - Crystallography

KW - Epitopes

KW - Fluorescent Dyes

KW - Humans

KW - Immunoglobulin Fab Fragments

KW - Mice

KW - Molecular Sequence Data

KW - Protein Structure, Tertiary

KW - Receptors, Adrenergic, beta-2

KW - Receptors, G-Protein-Coupled

KW - Recombinant Proteins

KW - Rhodamines

KW - Vaccination

U2 - 10.1038/nmeth1112

DO - 10.1038/nmeth1112

M3 - Journal article

C2 - 17952087

SN - 1548-7091

VL - 4

SP - 927

EP - 929

JO - Nature Methods

JF - Nature Methods

IS - 11

ER -

A monoclonal antibody for G protein-coupled receptor crystallography

Abstract

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