TY - JOUR
T1 - A microoptode array for fine-scale measurement of oxygen distribution
AU - Holst, Gerhard
AU - Glud, Ronnie N.
AU - Kühl, Michael
AU - Klimant, Ingo
PY - 1997/1/1
Y1 - 1997/1/1
N2 - A new microoptode array is presented that provides simultaneous measurement with eight oxygen microoptodes using a simple optical setup and a phase-angle detection principle. The measuring system consists of: (1) an optical unit with eight oxygen microoptodes, a special fiber-coupler array, optical filters, lenses, light sources (light-emitting diodes) and light detectors (photodiodes, photomultiplier tube); (2) a signal-processing unit with analog signal processing (phase-angle detection, filtering) and digital signal processing (control, data storage and display). The oxygen concentration is measured with tapered silica-glass fibers (tip diameter 20-30 μm) by the dynamic quenching of a luminophore. A phase-modulation technique is used to determine the phase-angle shift that is caused by the fluorescence lifetime when the indicator is excited sinusoidally. In a time multiplex mode each sensor signal is sampled. This multisensor array system is designed for the investigation of the oxygen distribution in biofilms and aquatic sediments. The new measuring system and first applications in artificial and natural systems are presented.
AB - A new microoptode array is presented that provides simultaneous measurement with eight oxygen microoptodes using a simple optical setup and a phase-angle detection principle. The measuring system consists of: (1) an optical unit with eight oxygen microoptodes, a special fiber-coupler array, optical filters, lenses, light sources (light-emitting diodes) and light detectors (photodiodes, photomultiplier tube); (2) a signal-processing unit with analog signal processing (phase-angle detection, filtering) and digital signal processing (control, data storage and display). The oxygen concentration is measured with tapered silica-glass fibers (tip diameter 20-30 μm) by the dynamic quenching of a luminophore. A phase-modulation technique is used to determine the phase-angle shift that is caused by the fluorescence lifetime when the indicator is excited sinusoidally. In a time multiplex mode each sensor signal is sampled. This multisensor array system is designed for the investigation of the oxygen distribution in biofilms and aquatic sediments. The new measuring system and first applications in artificial and natural systems are presented.
KW - Fluorescence lifetime sensing
KW - Fluorescence quenching
KW - Microoptodes
KW - Oxygen microsensors
KW - Oxygen optodes
KW - Phase modulation
UR - http://www.scopus.com/inward/record.url?scp=0030646652&partnerID=8YFLogxK
U2 - 10.1016/S0925-4005(97)80181-5
DO - 10.1016/S0925-4005(97)80181-5
M3 - Journal article
AN - SCOPUS:0030646652
SN - 0925-4005
VL - 38
SP - 122
EP - 129
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
IS - 1-3
ER -