A membrane trafficking pathway regulated by the plant-specific RAB GTPase ARA6

TY - JOUR

T1 - A membrane trafficking pathway regulated by the plant-specific RAB GTPase ARA6

AU - Ebine, Kazou

AU - Fujimoto, Masaru

AU - Okatani, Yusuke

AU - Nishiyama, Tomoaki

AU - Goh, Tatsuaki

AU - Ito, Emi

AU - Dainobu, Tomoko

AU - Nishitani, Aiko

AU - Uemura, Tomohiro

AU - Sato, Masa H.

AU - Thordal-Christensen, Hans

AU - Tsutsumi, Nobuhiro

AU - Nakano, Akihiko

AU - Ueda, Takashi

PY - 2011/7

Y1 - 2011/7

N2 - Endosomal trafficking plays an integral role in various eukaryotic cell activities and serves as a basis for higher-order functions in multicellular organisms. An understanding of the importance of endosomal trafficking in plants is rapidly developing, but its molecular mechanism is mostly unknown. Several key regulators of endosomal trafficking, including RAB5, which regulates diverse endocytic events in animal cells, are highly conserved. However, the identification of lineage-specific regulators in eukaryotes indicates that endosomal trafficking is diversified according to distinct body plans and lifestyles. In addition to orthologues of metazoan RAB5, land plants possess a unique RAB5 molecule, which is one of the most prominent features of plant RAB GTPase organization. Plants have also evolved a unique repertoire of SNAREs, the most distinctive of which are diverse VAMP7-related longins, including plant-unique VAMP72 derivatives. Here, we demonstrate that a plant-unique RAB5 protein, ARA6, acts in an endosomal trafficking pathway in Arabidopsis thaliana. ARA6 modulates the assembly of a distinct SNARE complex from conventional RAB5, and has a functional role in the salinity stress response. Our results indicate that plants possess a unique endosomal trafficking network and provide the first indication of a functional link between a specific RAB and a specific SNARE complex in plants.

AB - Endosomal trafficking plays an integral role in various eukaryotic cell activities and serves as a basis for higher-order functions in multicellular organisms. An understanding of the importance of endosomal trafficking in plants is rapidly developing, but its molecular mechanism is mostly unknown. Several key regulators of endosomal trafficking, including RAB5, which regulates diverse endocytic events in animal cells, are highly conserved. However, the identification of lineage-specific regulators in eukaryotes indicates that endosomal trafficking is diversified according to distinct body plans and lifestyles. In addition to orthologues of metazoan RAB5, land plants possess a unique RAB5 molecule, which is one of the most prominent features of plant RAB GTPase organization. Plants have also evolved a unique repertoire of SNAREs, the most distinctive of which are diverse VAMP7-related longins, including plant-unique VAMP72 derivatives. Here, we demonstrate that a plant-unique RAB5 protein, ARA6, acts in an endosomal trafficking pathway in Arabidopsis thaliana. ARA6 modulates the assembly of a distinct SNARE complex from conventional RAB5, and has a functional role in the salinity stress response. Our results indicate that plants possess a unique endosomal trafficking network and provide the first indication of a functional link between a specific RAB and a specific SNARE complex in plants.

U2 - 10.1038/ncb2270

DO - 10.1038/ncb2270

M3 - Letter

C2 - 21666683

SN - 1465-7392

VL - 13

SP - 853

EP - 860

JO - Nature Cell Biology

JF - Nature Cell Biology

IS - 7

ER -