@article{320f9590712e11de8bc9000ea68e967b,
title = "A fluorescent substrate for carbon-phosphorus lyase: towards the pathway for organophosphonate metabolism in bacteria",
abstract = "Many species of bacteria can use naturally occurring organophosphonates as a source of metabolic phosphate by cleaving the carbon-phosphorus bond with a multi-enzyme pathway collectively called carbon-phosphorus lyase (CP-lyase). Very little is known about the fate of organophosphonates entering this pathway. In order to detect metabolic intermediates we have synthesized a fluorescently labelled organophosphonate and show that this is a viable substrate for the CP-lyase pathway in Escherichia coli and that the expected product of CP-bond cleavage is formed. The in vivo competence of one potential metabolic intermediate, 1-ethylphosphonate-a-d-ribofuranose, is also demonstrated.",
author = "Shu-Mei He and Yan Lou and Bjarne Hove-Jensen and Zechel, {David L.}",
year = "2009",
doi = "10.1016/j.bmcl.2009.08.035",
language = "English",
volume = "19",
pages = "5954--5957",
journal = "Bioorganic & Medicinal Chemistry Letters",
issn = "0960-894X",
publisher = "Pergamon Press",
number = "20",
}