A fluorescence polarization based screening assay for identification of small molecule inhibitors of the PICK1 PDZ domain

Keyphrases

• Fluorescence Polarization 100%
• PDZ Domain 100%
• Screening Assays 100%
• PICK1 100%
• Small Molecule Inhibitors 100%
• Apparent Affinity 28%
• Postsynaptic Density Protein 95 (PSD-95) 28%
• Neuropathic Pain 14%
• GST Fusion Protein 14%
• Purified Proteins 14%
• GluR2 14%
• Living Cells 14%
• Zonula occludens-1 (ZO-1) 14%
• Fluorescence Polarization Assay 14%
• Fast Screening 14%
• C-terminus 14%
• Binding Experiment 14%
• Transfer Test 14%
• Discs Large 14%
• Dopamine Transporter 14%
• Domain-domain Interaction 14%
• Highly Reliable 14%
• Single-molecule Fluorescence Resonance Energy Transfer (smFRET) 14%
• Pullout 14%
• Competition Binding 14%
• Fluorescence Assay 14%
• Addiction 14%
• Cancer Pain 14%
• Putative Targets 14%

Biochemistry, Genetics and Molecular Biology

• PDZ Domain 100%
• Kinase 100%
• Phosphotransferase 100%
• Small Molecule 100%
• C-Terminus 42%
• Glutamate Receptor 14%
• Dopamine Transporter 14%
• Chimeric Protein 14%
• Amino Methyl Phosphonic Acid 14%
• Binding Competition 14%
• Fluorescence Resonance Energy Transfer 14%
• Solution and Solubility 14%

Immunology and Microbiology

• PDZ Domain 100%
• Carboxy Terminal Sequence 60%
• Binding Competition 20%
• Energy Transfer 20%
• Peptides 20%
• Solution and Solubility 20%