A draft genome assembly of the solar-powered sea slug Elysia chlorotica

Huimin Cai; Qiye Li; Xiaodong Fang; Ji Li; Nicholas E Curtis; Andreas Altenburger; Tomoko Shibata; Mingji Feng; Taro Maeda; Julie A Schwartz; Shuji Shigenobu; Nina Lundholm; Tomoaki Nishiyama; Huanming Yang; Mitsuyasu Hasebe; Shuaicheng Li; Sidney K Pierce; Jian Wang

doi:10.1038/sdata.2019.22

A draft genome assembly of the solar-powered sea slug Elysia chlorotica

Huimin Cai, Qiye Li, Xiaodong Fang, Ji Li, Nicholas E Curtis, Andreas Altenburger, Tomoko Shibata, Mingji Feng, Taro Maeda, Julie A Schwartz, Shuji Shigenobu, Nina Lundholm, Tomoaki Nishiyama, Huanming Yang, Mitsuyasu Hasebe, Shuaicheng Li, Sidney K Pierce, Jian Wang

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Abstract

Elysia chlorotica, a sacoglossan sea slug found off the East Coast of the United States, is well-known for its ability to sequester chloroplasts from its algal prey and survive by photosynthesis for up to 12 months in the absence of food supply. Here we present a draft genome assembly of E. chlorotica that was generated using a hybrid assembly strategy with Illumina short reads and PacBio long reads. The genome assembly comprised 9,989 scaffolds, with a total length of 557 Mb and a scaffold N50 of 442 kb. BUSCO assessment indicated that 93.3% of the expected metazoan genes were completely present in the genome assembly. Annotation of the E. chlorotica genome assembly identified 176 Mb (32.6%) of repetitive sequences and a total of 24,980 protein-coding genes. We anticipate that the annotated draft genome assembly of the E. chlorotica sea slug will promote the investigation of sacoglossan genetics, evolution, and particularly, the genetic signatures accounting for the long-term functioning of algal chloroplasts in an animal.

Originalsprog	Engelsk
Artikelnummer	190022
Tidsskrift	Scientific Data
Vol/bind	6
Antal sider	13
ISSN	2052-4463
DOI	https://doi.org/10.1038/sdata.2019.22
Status	Udgivet - 19 feb. 2019

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10.1038/sdata.2019.22Licens: CC BY

https://www.nature.com/articles/sdata201922.pdfLicens: CC BY

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Cai, H., Li, Q., Fang, X., Li, J., Curtis, N. E., Altenburger, A., Shibata, T., Feng, M., Maeda, T., Schwartz, J. A., Shigenobu, S., Lundholm, N., Nishiyama, T., Yang, H., Hasebe, M., Li, S., Pierce, S. K., & Wang, J. (2019). A draft genome assembly of the solar-powered sea slug Elysia chlorotica. Scientific Data, 6, Artikel 190022. https://doi.org/10.1038/sdata.2019.22

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title = "A draft genome assembly of the solar-powered sea slug Elysia chlorotica",

abstract = "Elysia chlorotica, a sacoglossan sea slug found off the East Coast of the United States, is well-known for its ability to sequester chloroplasts from its algal prey and survive by photosynthesis for up to 12 months in the absence of food supply. Here we present a draft genome assembly of E. chlorotica that was generated using a hybrid assembly strategy with Illumina short reads and PacBio long reads. The genome assembly comprised 9,989 scaffolds, with a total length of 557 Mb and a scaffold N50 of 442 kb. BUSCO assessment indicated that 93.3% of the expected metazoan genes were completely present in the genome assembly. Annotation of the E. chlorotica genome assembly identified 176 Mb (32.6%) of repetitive sequences and a total of 24,980 protein-coding genes. We anticipate that the annotated draft genome assembly of the E. chlorotica sea slug will promote the investigation of sacoglossan genetics, evolution, and particularly, the genetic signatures accounting for the long-term functioning of algal chloroplasts in an animal.",

author = "Huimin Cai and Qiye Li and Xiaodong Fang and Ji Li and Curtis, {Nicholas E} and Andreas Altenburger and Tomoko Shibata and Mingji Feng and Taro Maeda and Schwartz, {Julie A} and Shuji Shigenobu and Nina Lundholm and Tomoaki Nishiyama and Huanming Yang and Mitsuyasu Hasebe and Shuaicheng Li and Pierce, {Sidney K} and Jian Wang",

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T1 - A draft genome assembly of the solar-powered sea slug Elysia chlorotica

AU - Cai, Huimin

AU - Li, Qiye

AU - Fang, Xiaodong

AU - Li, Ji

AU - Curtis, Nicholas E

AU - Altenburger, Andreas

AU - Shibata, Tomoko

AU - Feng, Mingji

AU - Maeda, Taro

AU - Schwartz, Julie A

AU - Shigenobu, Shuji

AU - Lundholm, Nina

AU - Nishiyama, Tomoaki

AU - Yang, Huanming

AU - Hasebe, Mitsuyasu

AU - Li, Shuaicheng

AU - Pierce, Sidney K

AU - Wang, Jian

PY - 2019/2/19

Y1 - 2019/2/19

N2 - Elysia chlorotica, a sacoglossan sea slug found off the East Coast of the United States, is well-known for its ability to sequester chloroplasts from its algal prey and survive by photosynthesis for up to 12 months in the absence of food supply. Here we present a draft genome assembly of E. chlorotica that was generated using a hybrid assembly strategy with Illumina short reads and PacBio long reads. The genome assembly comprised 9,989 scaffolds, with a total length of 557 Mb and a scaffold N50 of 442 kb. BUSCO assessment indicated that 93.3% of the expected metazoan genes were completely present in the genome assembly. Annotation of the E. chlorotica genome assembly identified 176 Mb (32.6%) of repetitive sequences and a total of 24,980 protein-coding genes. We anticipate that the annotated draft genome assembly of the E. chlorotica sea slug will promote the investigation of sacoglossan genetics, evolution, and particularly, the genetic signatures accounting for the long-term functioning of algal chloroplasts in an animal.

AB - Elysia chlorotica, a sacoglossan sea slug found off the East Coast of the United States, is well-known for its ability to sequester chloroplasts from its algal prey and survive by photosynthesis for up to 12 months in the absence of food supply. Here we present a draft genome assembly of E. chlorotica that was generated using a hybrid assembly strategy with Illumina short reads and PacBio long reads. The genome assembly comprised 9,989 scaffolds, with a total length of 557 Mb and a scaffold N50 of 442 kb. BUSCO assessment indicated that 93.3% of the expected metazoan genes were completely present in the genome assembly. Annotation of the E. chlorotica genome assembly identified 176 Mb (32.6%) of repetitive sequences and a total of 24,980 protein-coding genes. We anticipate that the annotated draft genome assembly of the E. chlorotica sea slug will promote the investigation of sacoglossan genetics, evolution, and particularly, the genetic signatures accounting for the long-term functioning of algal chloroplasts in an animal.

U2 - 10.1038/sdata.2019.22

DO - 10.1038/sdata.2019.22

M3 - Journal article

C2 - 30778257

SN - 2052-4463

VL - 6

JO - Scientific Data

JF - Scientific Data

M1 - 190022

ER -

A draft genome assembly of the solar-powered sea slug Elysia chlorotica

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