TY - JOUR
T1 - A calorimetric study of phosphocholine membranes mixed with desmopressin and its diacylated prodrug derivative (DPP)
AU - Pedersen, Tina B.
AU - Frokjaer, Sven
AU - Mouritsen, Ole G.
AU - Jørgensen, Kent
PY - 2002/2/21
Y1 - 2002/2/21
N2 - The influence of the water-soluble peptide, desmopressin (DDAVP) and its dipalmitoylated prodrug derivative (DPP) on the thermal behaviour of three different saturated phosphatidylcholine lipid membranes was investigated by differential scanning calorimetry. For lipid membranes composed of dimyristoyl, dipalmitoyl and distearoyl phosphatidylcholines the addition of DDAVP at concentrations of up to 10 mol% resulted in an insignificant change in the thermodynamic phase behaviour. In contrast, the dipalmitoylated DPP prodrug caused major changes on the lipid membrane phase behaviour manifested as a drastic decrease in the heat capacity peak height and a concomitant broadening of the main phase transition as well as a decrease in the transition enthalpy. In addition, the main phase transition temperature was slightly decreased and the pre-transition of the three phosphatidylcholines was abolished when DPP was present.
AB - The influence of the water-soluble peptide, desmopressin (DDAVP) and its dipalmitoylated prodrug derivative (DPP) on the thermal behaviour of three different saturated phosphatidylcholine lipid membranes was investigated by differential scanning calorimetry. For lipid membranes composed of dimyristoyl, dipalmitoyl and distearoyl phosphatidylcholines the addition of DDAVP at concentrations of up to 10 mol% resulted in an insignificant change in the thermodynamic phase behaviour. In contrast, the dipalmitoylated DPP prodrug caused major changes on the lipid membrane phase behaviour manifested as a drastic decrease in the heat capacity peak height and a concomitant broadening of the main phase transition as well as a decrease in the transition enthalpy. In addition, the main phase transition temperature was slightly decreased and the pre-transition of the three phosphatidylcholines was abolished when DPP was present.
KW - Acylated peptide
KW - Differential scanning calorimetry
KW - Peptide-membrane interaction
UR - http://www.scopus.com/inward/record.url?scp=0037148677&partnerID=8YFLogxK
U2 - 10.1016/S0378-5173(01)00946-2
DO - 10.1016/S0378-5173(01)00946-2
M3 - Journal article
C2 - 11897424
AN - SCOPUS:0037148677
SN - 0378-5173
VL - 233
SP - 199
EP - 206
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
IS - 1-2
ER -