A biocompatible micro cell culture chamber (microCCC) for the culturing and on-line monitoring of eukaryote cells

TY - JOUR

T1 - A biocompatible micro cell culture chamber (microCCC) for the culturing and on-line monitoring of eukaryote cells

AU - Stangegaard, Michael

AU - Petronis, Sarunas

AU - Jørgensen, A M

AU - Christensen, C B V

AU - Dufva, Martin

PY - 2006/8

Y1 - 2006/8

N2 - We have previously shown that a polymeric (PMMA) chip with medium perfusion and integrated heat regulation provides sufficiently precise heat regulation, pH-control and medium exchange to support cell growth for weeks. However, it was unclear how closely the cells cultured in the chip resembled cells cultured in the culture flask. In the current study, gene expression profiles of cells cultured in the chip were compared with gene expression profiles of cells cultured in culture flasks. The results showed that there were only two genes that were differently expressed in cells grown in the cell culture chip compared to cell culture flasks. The cell culture chip could without further modification support cell growth of two other cell lines. Light coming from the microscope lamp during optical recordings of the cells was the only external factor identified, that could have a negative effect on cell survival. Low grade light exposure was however compatible with optical recordings as well as cell viability. These results strongly indicate that a cell culture chip could be constructed that allowed for on-line optical recording of cellular events without affecting the cell culturing condition compared to cell cultured in culture flasks incubated in a dark and CO2 conditioned incubator.

AB - We have previously shown that a polymeric (PMMA) chip with medium perfusion and integrated heat regulation provides sufficiently precise heat regulation, pH-control and medium exchange to support cell growth for weeks. However, it was unclear how closely the cells cultured in the chip resembled cells cultured in the culture flask. In the current study, gene expression profiles of cells cultured in the chip were compared with gene expression profiles of cells cultured in culture flasks. The results showed that there were only two genes that were differently expressed in cells grown in the cell culture chip compared to cell culture flasks. The cell culture chip could without further modification support cell growth of two other cell lines. Light coming from the microscope lamp during optical recordings of the cells was the only external factor identified, that could have a negative effect on cell survival. Low grade light exposure was however compatible with optical recordings as well as cell viability. These results strongly indicate that a cell culture chip could be constructed that allowed for on-line optical recording of cellular events without affecting the cell culturing condition compared to cell cultured in culture flasks incubated in a dark and CO2 conditioned incubator.

KW - Caco-2 Cells

KW - Cell Culture Techniques

KW - Cell Survival

KW - Gene Expression Profiling

KW - Gene Expression Regulation

KW - Hela Cells

KW - Humans

KW - Microfluidic Analytical Techniques

KW - Reverse Transcriptase Polymerase Chain Reaction

U2 - 10.1039/b603379b

DO - 10.1039/b603379b

M3 - Journal article

C2 - 16874376

SN - 1473-0197

VL - 6

SP - 1045

EP - 1051

JO - Lab on a Chip

JF - Lab on a Chip

IS - 8

ER -