TY - JOUR
T1 - A bacterial haloalkane dehalogenase gene as a negative selectable marker in Arabidopsis
AU - Næsted, Henrik
AU - Fennema, M.
AU - Hao, L.
AU - Andersen, M.
AU - Janssen, D.B.
AU - Mundy, John Williams
PY - 1999
Y1 - 1999
N2 - The dhlA gene of Xanthobacter autotrophicus GJ10 encodes a dehalogenase which hydrolyzes dihalo- alkanes, such as 1,2-dichloroethane (DCE), to a halo- genated alcohol and an inorganic halide ( Janssen et al. 1994 , Annu. Rev. Microbiol. 48, 163-191). In Xanthobacter, these alcohols are further catabolized by alcohol and aldehyde dehydrogenase activities, and by the product of the dhlB gene to a second halide and a hydroxyacid. The intermediate halogenated alcohols and, in particular, the aldehydes are more toxic than the haloalkane substrates or the pathway products. We show here that plants, including Arabidopsis, tobacco, oil seed rape and rice, do not express detectable haloalkane dehalogenase activities, and that wild-type Arabidopsis grows in the presence of DCE. In contrast, DCE applied as a volatile can be used to select on plates or in soil transgenic Arabidopsis which express dhlA. The dhlA marker therefore provides haloalkane dehalogenase reporter activity and substrate dependent negative selection in transgenic plants.
AB - The dhlA gene of Xanthobacter autotrophicus GJ10 encodes a dehalogenase which hydrolyzes dihalo- alkanes, such as 1,2-dichloroethane (DCE), to a halo- genated alcohol and an inorganic halide ( Janssen et al. 1994 , Annu. Rev. Microbiol. 48, 163-191). In Xanthobacter, these alcohols are further catabolized by alcohol and aldehyde dehydrogenase activities, and by the product of the dhlB gene to a second halide and a hydroxyacid. The intermediate halogenated alcohols and, in particular, the aldehydes are more toxic than the haloalkane substrates or the pathway products. We show here that plants, including Arabidopsis, tobacco, oil seed rape and rice, do not express detectable haloalkane dehalogenase activities, and that wild-type Arabidopsis grows in the presence of DCE. In contrast, DCE applied as a volatile can be used to select on plates or in soil transgenic Arabidopsis which express dhlA. The dhlA marker therefore provides haloalkane dehalogenase reporter activity and substrate dependent negative selection in transgenic plants.
U2 - 10.1046/j.1365-313X.1999.00477.x
DO - 10.1046/j.1365-313X.1999.00477.x
M3 - Journal article
SN - 0960-7412
VL - 18
SP - 571
EP - 576
JO - Plant Journal
JF - Plant Journal
IS - 5
ER -