TY - JOUR
T1 - A BAC transgenic Hes1-EGFP reporter reveals novel expression domains in mouse embryos
AU - Klinck, Rasmus
AU - Füchtbauer, Ernst-Martin
AU - Ahnfelt-Rønne, Jonas
AU - Serup, Palle
AU - Jensen, Jan Nygaard
AU - Jørgensen, Mette Christine
N1 - Copyright © 2011 Elsevier B.V. All rights reserved.
PY - 2011/10
Y1 - 2011/10
N2 - Expression of the basic helix-loop-helix factor Hairy and Enhancer of Split-1 (Hes1) is required for normal development of a number of tissues during embryonic development. Depending on context, Hes1 may act as a Notch signalling effector which promotes the undifferentiated and proliferative state of progenitor cells, but increasing evidence also points to Notch independent regulation of Hes1 expression. Here we use high resolution confocal scanning of EGFP in a novel BAC transgenic mouse reporter line, Tg(Hes1-EGFP)(1Hri), to analyse Hes1 expression from embryonic day 7.0 (e7.0). Our data recapitulates some previous observations on Hes1 expression and suggests new, hitherto unrecognised expression domains including expression in the definitive endoderm at early somite stages before gut tube closure and thus preceding organogenesis. This mouse line will be a valuable tool for studies addressing the role of Hes1 in a number of different research areas including organ specification, development and regeneration.
AB - Expression of the basic helix-loop-helix factor Hairy and Enhancer of Split-1 (Hes1) is required for normal development of a number of tissues during embryonic development. Depending on context, Hes1 may act as a Notch signalling effector which promotes the undifferentiated and proliferative state of progenitor cells, but increasing evidence also points to Notch independent regulation of Hes1 expression. Here we use high resolution confocal scanning of EGFP in a novel BAC transgenic mouse reporter line, Tg(Hes1-EGFP)(1Hri), to analyse Hes1 expression from embryonic day 7.0 (e7.0). Our data recapitulates some previous observations on Hes1 expression and suggests new, hitherto unrecognised expression domains including expression in the definitive endoderm at early somite stages before gut tube closure and thus preceding organogenesis. This mouse line will be a valuable tool for studies addressing the role of Hes1 in a number of different research areas including organ specification, development and regeneration.
KW - Animals
KW - Basic Helix-Loop-Helix Transcription Factors
KW - Chromosomes, Artificial, Bacterial
KW - Endoderm
KW - Gene Expression Regulation, Developmental
KW - Green Fluorescent Proteins
KW - Homeodomain Proteins
KW - Mice
KW - Mice, Transgenic
KW - Organogenesis
KW - Somites
U2 - 10.1016/j.gep.2011.06.004
DO - 10.1016/j.gep.2011.06.004
M3 - Journal article
C2 - 21745596
SN - 1567-133X
VL - 11
SP - 415
EP - 426
JO - Gene Expression Patterns
JF - Gene Expression Patterns
IS - 7
ER -