a-Adrenergic vasoconstrictor responsiveness is preserved in the heated human leg

David M Keller; Mikael Sander; Bente Merete Stallknecht; Craig G Crandall

doi:10.1113/jphysiol.2010.194506

a-Adrenergic vasoconstrictor responsiveness is preserved in the heated human leg

David M Keller, Mikael Sander, Bente Merete Stallknecht, Craig G Crandall

Torekov Group

36 Citationer (Scopus)

Abstract

This study tested the hypothesis that passive leg heating attenuates α-adrenergic vasoconstriction within that limb. Femoral blood flow (FBF, femoral artery ultrasound Doppler) and femoral vascular conductance (FVC, FBF/mean arterial blood pressure), as well as calf muscle blood flow (CalfBF, ¹³³xenon) and calf vascular conductance (CalfVC) were measured during intra-arterial infusion of an α₁-adrenoreceptor agonist, phenylephrine (PE, 0.025 to 0.8 μg kg^-1 min^-1) and an α₂-adrenoreceptor agonist, BHT-933 (1.0 to 10 μg kg^-1 min^-1) during normothermia and passive leg heating (water-perfused pant leg). Passive leg heating (∼46°C water temperature) increased FVC from 4.5 ± 0.5 to 11.9 ± 1.3 ml min^-1 mmHg^-1 (P < 0.001). Interestingly, CalfBF (1.8 ± 0.2 vs. 2.8 ± 0.3 ml min^-1 (100 g)^-1) and CalfVC (2.0 ± 0.3 vs. 3.9 ± 0.5 ml min^-1 (100 g)^-1 mmHg^-1× 100) were also increased by this perturbation (P < 0.05 for both). Infusion of PE and BHT-933 resulted in greater absolute decreases in FVC during leg heating compared to normothermic conditions (maximal decreases in FVC during heating vs. normothermia: PE: 7.8 ± 1.1 vs. 2.8 ± 0.5 ml min^-1 mmHg^-1; BHT-933: 8.6 ± 1.7 vs. 2.1 ± 0.4 ml min^-1 mmHg^-1; P < 0.01 for both). However, the nadir FVC during drug infusion was higher during passive leg heating compared to normothermic conditions (FVC at highest dose of respective drugs during heating vs. normothermic conditions: PE: 3.7 ± 0.4 vs. 2.0 ± 0.3 ml min^-1 mmHg^-1; BHT-933: 3.8 ± 0.2 vs. 2.1 ± 0.3 ml min^-1 mmHg^-1; P < 0.001 for both). Leg heating did not alter the responsiveness of CalfBF or CalfVC to either PE or BHT-933. Taken together, these observations suggest that local heating does not decrease α-adrenergic responsiveness. However, heat-induced vasodilatation opposes α-adrenergic vasoconstriction. Furthermore, passive heating of a limb causes not only an increase in skin blood flow but also in muscle blood flow.

Originalsprog	Engelsk
Tidsskrift	Journal of Physiology
Vol/bind	588
Udgave nummer	Pt 19
Sider (fra-til)	3799-808
Antal sider	10
ISSN	0022-3751
DOI	https://doi.org/10.1113/jphysiol.2010.194506
Status	Udgivet - 1 okt. 2010

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10.1113/jphysiol.2010.194506

Citationsformater

@article{77cb8984fad6468996d13a861f8e210e,

title = "a-Adrenergic vasoconstrictor responsiveness is preserved in the heated human leg",

abstract = "This study tested the hypothesis that passive leg heating attenuates α-adrenergic vasoconstriction within that limb. Femoral blood flow (FBF, femoral artery ultrasound Doppler) and femoral vascular conductance (FVC, FBF/mean arterial blood pressure), as well as calf muscle blood flow (CalfBF, 133xenon) and calf vascular conductance (CalfVC) were measured during intra-arterial infusion of an α1-adrenoreceptor agonist, phenylephrine (PE, 0.025 to 0.8 μg kg-1 min-1) and an α2-adrenoreceptor agonist, BHT-933 (1.0 to 10 μg kg-1 min-1) during normothermia and passive leg heating (water-perfused pant leg). Passive leg heating (∼46°C water temperature) increased FVC from 4.5 ± 0.5 to 11.9 ± 1.3 ml min-1 mmHg-1 (P < 0.001). Interestingly, CalfBF (1.8 ± 0.2 vs. 2.8 ± 0.3 ml min-1 (100 g)-1) and CalfVC (2.0 ± 0.3 vs. 3.9 ± 0.5 ml min-1 (100 g)-1 mmHg-1× 100) were also increased by this perturbation (P < 0.05 for both). Infusion of PE and BHT-933 resulted in greater absolute decreases in FVC during leg heating compared to normothermic conditions (maximal decreases in FVC during heating vs. normothermia: PE: 7.8 ± 1.1 vs. 2.8 ± 0.5 ml min-1 mmHg-1; BHT-933: 8.6 ± 1.7 vs. 2.1 ± 0.4 ml min-1 mmHg-1; P < 0.01 for both). However, the nadir FVC during drug infusion was higher during passive leg heating compared to normothermic conditions (FVC at highest dose of respective drugs during heating vs. normothermic conditions: PE: 3.7 ± 0.4 vs. 2.0 ± 0.3 ml min-1 mmHg-1; BHT-933: 3.8 ± 0.2 vs. 2.1 ± 0.3 ml min-1 mmHg-1; P < 0.001 for both). Leg heating did not alter the responsiveness of CalfBF or CalfVC to either PE or BHT-933. Taken together, these observations suggest that local heating does not decrease α-adrenergic responsiveness. However, heat-induced vasodilatation opposes α-adrenergic vasoconstriction. Furthermore, passive heating of a limb causes not only an increase in skin blood flow but also in muscle blood flow.",

keywords = "Adrenergic alpha-1 Receptor Agonists, Adrenergic alpha-2 Receptor Antagonists, Adrenergic alpha-Agonists, Adult, Azepines, Blood Pressure, Body Temperature, Dose-Response Relationship, Drug, Hot Temperature, Humans, Leg, Male, Muscle, Skeletal, Phenylephrine, Receptors, Adrenergic, alpha, Regional Blood Flow, Ultrasonography, Doppler, Vasoconstriction, Xenon Radioisotopes, Young Adult",

author = "Keller, {David M} and Mikael Sander and Stallknecht, {Bente Merete} and Crandall, {Craig G}",

year = "2010",

month = oct,

day = "1",

doi = "10.1113/jphysiol.2010.194506",

language = "English",

volume = "588",

pages = "3799--808",

journal = "Journal of Physiology",

issn = "0022-3751",

publisher = "Wiley-Blackwell",

number = "Pt 19",

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TY - JOUR

T1 - a-Adrenergic vasoconstrictor responsiveness is preserved in the heated human leg

AU - Keller, David M

AU - Sander, Mikael

AU - Stallknecht, Bente Merete

AU - Crandall, Craig G

PY - 2010/10/1

Y1 - 2010/10/1

N2 - This study tested the hypothesis that passive leg heating attenuates α-adrenergic vasoconstriction within that limb. Femoral blood flow (FBF, femoral artery ultrasound Doppler) and femoral vascular conductance (FVC, FBF/mean arterial blood pressure), as well as calf muscle blood flow (CalfBF, 133xenon) and calf vascular conductance (CalfVC) were measured during intra-arterial infusion of an α1-adrenoreceptor agonist, phenylephrine (PE, 0.025 to 0.8 μg kg-1 min-1) and an α2-adrenoreceptor agonist, BHT-933 (1.0 to 10 μg kg-1 min-1) during normothermia and passive leg heating (water-perfused pant leg). Passive leg heating (∼46°C water temperature) increased FVC from 4.5 ± 0.5 to 11.9 ± 1.3 ml min-1 mmHg-1 (P < 0.001). Interestingly, CalfBF (1.8 ± 0.2 vs. 2.8 ± 0.3 ml min-1 (100 g)-1) and CalfVC (2.0 ± 0.3 vs. 3.9 ± 0.5 ml min-1 (100 g)-1 mmHg-1× 100) were also increased by this perturbation (P < 0.05 for both). Infusion of PE and BHT-933 resulted in greater absolute decreases in FVC during leg heating compared to normothermic conditions (maximal decreases in FVC during heating vs. normothermia: PE: 7.8 ± 1.1 vs. 2.8 ± 0.5 ml min-1 mmHg-1; BHT-933: 8.6 ± 1.7 vs. 2.1 ± 0.4 ml min-1 mmHg-1; P < 0.01 for both). However, the nadir FVC during drug infusion was higher during passive leg heating compared to normothermic conditions (FVC at highest dose of respective drugs during heating vs. normothermic conditions: PE: 3.7 ± 0.4 vs. 2.0 ± 0.3 ml min-1 mmHg-1; BHT-933: 3.8 ± 0.2 vs. 2.1 ± 0.3 ml min-1 mmHg-1; P < 0.001 for both). Leg heating did not alter the responsiveness of CalfBF or CalfVC to either PE or BHT-933. Taken together, these observations suggest that local heating does not decrease α-adrenergic responsiveness. However, heat-induced vasodilatation opposes α-adrenergic vasoconstriction. Furthermore, passive heating of a limb causes not only an increase in skin blood flow but also in muscle blood flow.

AB - This study tested the hypothesis that passive leg heating attenuates α-adrenergic vasoconstriction within that limb. Femoral blood flow (FBF, femoral artery ultrasound Doppler) and femoral vascular conductance (FVC, FBF/mean arterial blood pressure), as well as calf muscle blood flow (CalfBF, 133xenon) and calf vascular conductance (CalfVC) were measured during intra-arterial infusion of an α1-adrenoreceptor agonist, phenylephrine (PE, 0.025 to 0.8 μg kg-1 min-1) and an α2-adrenoreceptor agonist, BHT-933 (1.0 to 10 μg kg-1 min-1) during normothermia and passive leg heating (water-perfused pant leg). Passive leg heating (∼46°C water temperature) increased FVC from 4.5 ± 0.5 to 11.9 ± 1.3 ml min-1 mmHg-1 (P < 0.001). Interestingly, CalfBF (1.8 ± 0.2 vs. 2.8 ± 0.3 ml min-1 (100 g)-1) and CalfVC (2.0 ± 0.3 vs. 3.9 ± 0.5 ml min-1 (100 g)-1 mmHg-1× 100) were also increased by this perturbation (P < 0.05 for both). Infusion of PE and BHT-933 resulted in greater absolute decreases in FVC during leg heating compared to normothermic conditions (maximal decreases in FVC during heating vs. normothermia: PE: 7.8 ± 1.1 vs. 2.8 ± 0.5 ml min-1 mmHg-1; BHT-933: 8.6 ± 1.7 vs. 2.1 ± 0.4 ml min-1 mmHg-1; P < 0.01 for both). However, the nadir FVC during drug infusion was higher during passive leg heating compared to normothermic conditions (FVC at highest dose of respective drugs during heating vs. normothermic conditions: PE: 3.7 ± 0.4 vs. 2.0 ± 0.3 ml min-1 mmHg-1; BHT-933: 3.8 ± 0.2 vs. 2.1 ± 0.3 ml min-1 mmHg-1; P < 0.001 for both). Leg heating did not alter the responsiveness of CalfBF or CalfVC to either PE or BHT-933. Taken together, these observations suggest that local heating does not decrease α-adrenergic responsiveness. However, heat-induced vasodilatation opposes α-adrenergic vasoconstriction. Furthermore, passive heating of a limb causes not only an increase in skin blood flow but also in muscle blood flow.

KW - Adrenergic alpha-1 Receptor Agonists

KW - Adrenergic alpha-2 Receptor Antagonists

KW - Adrenergic alpha-Agonists

KW - Adult

KW - Azepines

KW - Blood Pressure

KW - Body Temperature

KW - Dose-Response Relationship, Drug

KW - Hot Temperature

KW - Humans

KW - Leg

KW - Male

KW - Muscle, Skeletal

KW - Phenylephrine

KW - Receptors, Adrenergic, alpha

KW - Regional Blood Flow

KW - Ultrasonography, Doppler

KW - Vasoconstriction

KW - Xenon Radioisotopes

KW - Young Adult

U2 - 10.1113/jphysiol.2010.194506

DO - 10.1113/jphysiol.2010.194506

M3 - Journal article

C2 - 20693291

SN - 0022-3751

VL - 588

SP - 3799

EP - 3808

JO - Journal of Physiology

JF - Journal of Physiology

IS - Pt 19

ER -

a-Adrenergic vasoconstrictor responsiveness is preserved in the heated human leg

Abstract

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