β-Cyclodextrin as a mimetic of the natural GFP-chromophore environment

TY - JOUR

T1 - β-Cyclodextrin as a mimetic of the natural GFP-chromophore environment

AU - Cacciarini, Martina

AU - Nielsen, Mogens Brøndsted

AU - de Castro, Elisa Martinez

AU - Marinescu-Pedersen, Lavinia G

AU - Bols, Mikael

PY - 2012/2/22

Y1 - 2012/2/22

N2 - The green fluorescent protein (GFP) chromophore has been anchored to β-cyclodextrin (βCD) via a copper(I)-catalyzed azide-alkyne cycloaddition. The photophysical properties of this new GFP-CD derivative have been evaluated, showing the formation of a self-inclusion complex and enhancement of fluorescence of the GFP-chromophore covalently bound to the βCD. This enhancement of fluorescence by encapsulation and hence fixation of the chromophore in a binding pocket mimics the effect exerted by the natural protein environment.

AB - The green fluorescent protein (GFP) chromophore has been anchored to β-cyclodextrin (βCD) via a copper(I)-catalyzed azide-alkyne cycloaddition. The photophysical properties of this new GFP-CD derivative have been evaluated, showing the formation of a self-inclusion complex and enhancement of fluorescence of the GFP-chromophore covalently bound to the βCD. This enhancement of fluorescence by encapsulation and hence fixation of the chromophore in a binding pocket mimics the effect exerted by the natural protein environment.

U2 - 10.1016/j.tetlet.2011.12.053

DO - 10.1016/j.tetlet.2011.12.053

M3 - Journal article

SN - 0040-4039

VL - 53

SP - 973

EP - 976

JO - Tetrahedron Letters

JF - Tetrahedron Letters

IS - 8

ER -