α-MSH stimulates glucose uptake in mouse muscle and phosphorylates Rab-GTPase-activating protein TBC1D1 independently of AMPK

Cathrine Laustrup Møller; Rasmus Kjøbsted; Pablo J Enriori; Thomas Elbenhardt Jensen; Cecilia Garcia-Rudaz; Sara A Litwak; Kirsten Raun; Jørgen Wojtaszewski; Birgitte Schjellerup Wulff; Michael A Cowley

doi:10.1371/journal.pone.0157027

α-MSH stimulates glucose uptake in mouse muscle and phosphorylates Rab-GTPase-activating protein TBC1D1 independently of AMPK

Cathrine Laustrup Møller, Rasmus Kjøbsted, Pablo J Enriori, Thomas Elbenhardt Jensen, Cecilia Garcia-Rudaz, Sara A Litwak, Kirsten Raun, Jørgen Wojtaszewski, Birgitte Schjellerup Wulff, Michael A Cowley

August Krogh Sektionen for Molekylær Fysiologi

4 Citationer (Scopus)

103 Downloads (Pure)

Abstract

The melanocortin system includes five G-protein coupled receptors (family A) defined as MC1R-MC5R, which are stimulated by endogenous agonists derived from proopiomelanocortin (POMC). The melanocortin system has been intensely studied for its central actions in body weight and energy expenditure regulation, which are mainly mediated by MC4R. The pituitary gland is the source of various POMC-derived hormones released to the circulation, which raises the possibility that there may be actions of the melanocortins on peripheral energy homeostasis. In this study, we examined the molecular signaling pathway involved in α-MSH-stimulated glucose uptake in differentiated L6 myotubes and mouse muscle explants. In order to examine the involvement of AMPK, we investigate -MSH stimulation in both wild type and AMPK deficient mice. We found that -MSH significantly induces phosphorylation of TBC1 domain (TBC1D) family member 1 (S237 and T596), which is independent of upstream PKA and AMPK. We find no evidence to support that -MSH-stimulated glucose uptake involves TBC1D4 phosphorylation (T642 and S704) or GLUT4 translocation.

Originalsprog	Engelsk
Artikelnummer	e0157027
Tidsskrift	P L o S One
Vol/bind	11
Udgave nummer	7
Antal sider	18
ISSN	1932-6203
DOI	https://doi.org/10.1371/journal.pone.0157027
Status	Udgivet - jul. 2016

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10.1371/journal.pone.0157027Licens: CC BY

Møller et al_P L o S ONE_2016_Vol 11(7)_e0157027Forlagets udgivne version, 1,21 MBLicens: CC BY

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Møller, C. L., Kjøbsted, R., Enriori, P. J., Jensen, T. E., Garcia-Rudaz, C., Litwak, S. A., Raun, K., Wojtaszewski, J., Wulff, B. S., & Cowley, M. A. (2016). α-MSH stimulates glucose uptake in mouse muscle and phosphorylates Rab-GTPase-activating protein TBC1D1 independently of AMPK. P L o S One, 11(7), Artikel e0157027. https://doi.org/10.1371/journal.pone.0157027

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abstract = "The melanocortin system includes five G-protein coupled receptors (family A) defined as MC1R-MC5R, which are stimulated by endogenous agonists derived from proopiomelanocortin (POMC). The melanocortin system has been intensely studied for its central actions in body weight and energy expenditure regulation, which are mainly mediated by MC4R. The pituitary gland is the source of various POMC-derived hormones released to the circulation, which raises the possibility that there may be actions of the melanocortins on peripheral energy homeostasis. In this study, we examined the molecular signaling pathway involved in α-MSH-stimulated glucose uptake in differentiated L6 myotubes and mouse muscle explants. In order to examine the involvement of AMPK, we investigate -MSH stimulation in both wild type and AMPK deficient mice. We found that -MSH significantly induces phosphorylation of TBC1 domain (TBC1D) family member 1 (S237 and T596), which is independent of upstream PKA and AMPK. We find no evidence to support that -MSH-stimulated glucose uptake involves TBC1D4 phosphorylation (T642 and S704) or GLUT4 translocation.",

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AU - Møller, Cathrine Laustrup

AU - Kjøbsted, Rasmus

AU - Enriori, Pablo J

AU - Jensen, Thomas Elbenhardt

AU - Garcia-Rudaz, Cecilia

AU - Litwak, Sara A

AU - Raun, Kirsten

AU - Wojtaszewski, Jørgen

AU - Wulff, Birgitte Schjellerup

AU - Cowley, Michael A

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N2 - The melanocortin system includes five G-protein coupled receptors (family A) defined as MC1R-MC5R, which are stimulated by endogenous agonists derived from proopiomelanocortin (POMC). The melanocortin system has been intensely studied for its central actions in body weight and energy expenditure regulation, which are mainly mediated by MC4R. The pituitary gland is the source of various POMC-derived hormones released to the circulation, which raises the possibility that there may be actions of the melanocortins on peripheral energy homeostasis. In this study, we examined the molecular signaling pathway involved in α-MSH-stimulated glucose uptake in differentiated L6 myotubes and mouse muscle explants. In order to examine the involvement of AMPK, we investigate -MSH stimulation in both wild type and AMPK deficient mice. We found that -MSH significantly induces phosphorylation of TBC1 domain (TBC1D) family member 1 (S237 and T596), which is independent of upstream PKA and AMPK. We find no evidence to support that -MSH-stimulated glucose uptake involves TBC1D4 phosphorylation (T642 and S704) or GLUT4 translocation.

AB - The melanocortin system includes five G-protein coupled receptors (family A) defined as MC1R-MC5R, which are stimulated by endogenous agonists derived from proopiomelanocortin (POMC). The melanocortin system has been intensely studied for its central actions in body weight and energy expenditure regulation, which are mainly mediated by MC4R. The pituitary gland is the source of various POMC-derived hormones released to the circulation, which raises the possibility that there may be actions of the melanocortins on peripheral energy homeostasis. In this study, we examined the molecular signaling pathway involved in α-MSH-stimulated glucose uptake in differentiated L6 myotubes and mouse muscle explants. In order to examine the involvement of AMPK, we investigate -MSH stimulation in both wild type and AMPK deficient mice. We found that -MSH significantly induces phosphorylation of TBC1 domain (TBC1D) family member 1 (S237 and T596), which is independent of upstream PKA and AMPK. We find no evidence to support that -MSH-stimulated glucose uptake involves TBC1D4 phosphorylation (T642 and S704) or GLUT4 translocation.

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α-MSH stimulates glucose uptake in mouse muscle and phosphorylates Rab-GTPase-activating protein TBC1D1 independently of AMPK

Abstract

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