Additional file 6 of Evidence of steady-state fibroblast subtypes in the normal human breast as cells-of-origin for perturbed-state fibroblasts in breast cancer

Additional file 6. Figure S4a: CAFs lack osteogenic differentiation capacity in culture and in vivo. a) Bar diagram of quantification of osteogenic differentiation among iCAFs, myCAFs and bMSCs upon exposure to control medium (-) or osteogenic inducing medium (OIM, +) determined after staining with alizarin red for which representative micrographs are shown. For myCAFs and bMSCs error bars represent +/- SD of three biological repeats each in technical duplicate. For iCAFs the bars represent the mean +/- SD of technical duplicates. One-way ANOVA with Benjamini-Hochberg multiple test correction comparing myCAFs and bMSCs found significant matrix mineralization among bMSCs between OIM and CM and between myCAFs OIM and bMSCs OIM (asterisk indicates p