Additional file 5 of Evidence of steady-state fibroblast subtypes in the normal human breast as cells-of-origin for perturbed-state fibroblasts in breast cancer

Additional file 5. Figure S3: CAFlow are interlobular-like and CAFhigh are lobular-like. a) Micrographs of CAFlow and CAFhigh derived from CAF2 and CAF3 immunoperoxidase-stained against CD105, tenascin and CD26. Note that irrespective of origin, the staining profiles with respect to CD105 and tenascin in CAFlow and CAFhigh correspond to those of interlobular fibroblasts and lobular fibroblasts, respectively (for comparison see Fig. 1d and 3a). The absence of CD26 in both CAFs indicates that this marker of the interlobular fibroblast lineage is state-dependent. Scale bar = 100 μm. b) Heatmap depicting gene expression fold changes determined by RT-qPCR in lobular fibroblasts relative to interlobular fibroblasts, myCAFs relative to iCAFs and in CAFhigh relative to CAFlow derived from CAF2- and CAF3. Color key represents the log10-transformed fold changes. Numbers are p-values by Student’s unpaired t-test.