Additional file 4 of Evidence of steady-state fibroblast subtypes in the normal human breast as cells-of-origin for perturbed-state fibroblasts in breast cancer

Additional file 4. Figure S2: CAFlow and CAFhigh represent iCAFs and myCAFs, respectively. a) Heatmap depicting the expression level fold change of six iCAF and six myCAF genes measured by RT-qPCR in CAFhigh expressed relative to CAFlow derived from CAF1 (left), CAF2 (middle) and CAF3 (right). Color key represents the log10-transformed CAFhigh/CAFlow fold change. Numbers are the p-values by Student’s unpaired t-test. b) Micrographs of CAFlow (upper) and CAFhigh (lower) derived from CAF1 (left), CAF2 (middle) and CAF3 (right) immunoperoxidase-stained against α-sm actin (brown) and counterstained by hematoxylin for nuclei (blue). Irrespective of origin, CAFhigh exhibit prominent positive staining which is relatively infrequent in CAFlow. Scale bar = 100 μm.