Validation of a high-performance liquid chromatography/fluorescence detection method for the simultaneous quantification of fifteen polycyclic aromatic hydrocarbons

Åse Marie Hansen; I L Olsen; E Holst; O M Poulsen

Validation of a high-performance liquid chromatography/fluorescence detection method for the simultaneous quantification of fifteen polycyclic aromatic hydrocarbons

Åse Marie Hansen, I L Olsen, E Holst, O M Poulsen

Department of Public Health

30 Citations (Scopus)

Abstract

A high-performance liquid chromatography/fluorescence method using multiple wavelength shift for simultaneous quantification of different PAH compounds was developed. The new method was superior to the methods of DONG and GREENBERG [J. Liquid Chromatogr. 11, 1887-1905 (1988)] and WISE et al. [Polycyclic aromat. Hydrocarb. (in press)] with respect to sensitivity of detection of the majority of 15 PAH compounds, and in particular of naphthalene, acenaphthene, fluorene and benzo(b)fluoranthene. The method of validation analysis employed showed that the new method is in statistical balance meaning that no systematic errors, and only small unsystematic errors, could be demonstrated. Furthermore, the method had a good reproducibility and a high sensitivity.

Original language	English
Journal	Annals of Occupational Hygiene
Volume	35
Issue number	6
Pages (from-to)	603-11
Number of pages	9
ISSN	2398-7308
Publication status	Published - Dec 1991

Keywords

Air Pollutants, Occupational
Chromatography, High Pressure Liquid
Evaluation Studies as Topic
Fluorescent Dyes
Humans
Polycyclic Compounds
Reproducibility of Results

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Validation of a high-performance liquid chromatography/fluorescence detection method for the simultaneous quantification of fifteen polycyclic aromatic hydrocarbons. / Hansen, Åse Marie; Olsen, I L; Holst, E et al.

In: Annals of Occupational Hygiene, Vol. 35, No. 6, 12.1991, p. 603-11.

Research output: Contribution to journal › Journal article › Research › peer-review

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AU - Holst, E

AU - Poulsen, O M

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N2 - A high-performance liquid chromatography/fluorescence method using multiple wavelength shift for simultaneous quantification of different PAH compounds was developed. The new method was superior to the methods of DONG and GREENBERG [J. Liquid Chromatogr. 11, 1887-1905 (1988)] and WISE et al. [Polycyclic aromat. Hydrocarb. (in press)] with respect to sensitivity of detection of the majority of 15 PAH compounds, and in particular of naphthalene, acenaphthene, fluorene and benzo(b)fluoranthene. The method of validation analysis employed showed that the new method is in statistical balance meaning that no systematic errors, and only small unsystematic errors, could be demonstrated. Furthermore, the method had a good reproducibility and a high sensitivity.

AB - A high-performance liquid chromatography/fluorescence method using multiple wavelength shift for simultaneous quantification of different PAH compounds was developed. The new method was superior to the methods of DONG and GREENBERG [J. Liquid Chromatogr. 11, 1887-1905 (1988)] and WISE et al. [Polycyclic aromat. Hydrocarb. (in press)] with respect to sensitivity of detection of the majority of 15 PAH compounds, and in particular of naphthalene, acenaphthene, fluorene and benzo(b)fluoranthene. The method of validation analysis employed showed that the new method is in statistical balance meaning that no systematic errors, and only small unsystematic errors, could be demonstrated. Furthermore, the method had a good reproducibility and a high sensitivity.

KW - Air Pollutants, Occupational

KW - Chromatography, High Pressure Liquid

KW - Evaluation Studies as Topic

KW - Fluorescent Dyes

KW - Humans

KW - Polycyclic Compounds

KW - Reproducibility of Results

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C2 - 1768009

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VL - 35

SP - 603

EP - 611

JO - Annals of Occupational Hygiene

JF - Annals of Occupational Hygiene

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ER -

Validation of a high-performance liquid chromatography/fluorescence detection method for the simultaneous quantification of fifteen polycyclic aromatic hydrocarbons

Abstract

Keywords

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