Ultrastructure of sheep primordial follicles cultured in the presence of indol acetic acid, EGF, and FSH

Evelyn Rabelo Andrade; Poul Hyttel; Fernanda Da Cruz  Landim-Alvarenga; José Roberto Viana Silva; Amauri Alcindo Alfieri; Marcelo Marcondes Seneda; José Ricardo Figueiredo; Ricardo Toniolli

doi:10.4061/2011/670987

Ultrastructure of sheep primordial follicles cultured in the presence of indol acetic acid, EGF, and FSH

Evelyn Rabelo Andrade, Poul Hyttel, Fernanda Da Cruz Landim-Alvarenga, José Roberto Viana Silva, Amauri Alcindo Alfieri, Marcelo Marcondes Seneda, José Ricardo Figueiredo, Ricardo Toniolli

6 Citations (Scopus)

Abstract

The aim of this study was to investigate the ultrastructural characteristics of primordial follicles after culturing of sheep ovarian cortical slices in the presence of indol acetic acid (IAA), Epidermal Growth Factor (EGF), and FSH. To evaluate ultrastructure of primordial follicles cultured in MEM (control) or in MEM containing IAA, EGF, and FSH, fragments of cultured tissue were processes for transmission electron microscopy. Except in the control, primordial follicles cultured in supplemented media for 6¿d were ultrastructurally normal. They had oocyte with intact nucleus and the cytoplasm contained heterogeneous-sized lipid droplets and numerous round or elongated mitochondria with intact parallel cristae were observed. Rough endoplasmic reticulum (RER) was rarely found. The granulosa cells cytoplasm contained a great number of mitochondria and abundant RER. In conclusion, the presence of IAA, EGF, and FSH helped to maintain ultrastructural integrity of sheep primordial follicles cultured in vitro.

Original language	English
Journal	Veterinary Medicine International
Volume	2011
Number of pages	7
ISSN	2042-0048
DOIs	https://doi.org/10.4061/2011/670987
Publication status	Published - 2011

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10.4061/2011/670987

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@article{a61311f7f7254764ba821a395cd08411,

title = "Ultrastructure of sheep primordial follicles cultured in the presence of indol acetic acid, EGF, and FSH",

abstract = "The aim of this study was to investigate the ultrastructural characteristics of primordial follicles after culturing of sheep ovarian cortical slices in the presence of indol acetic acid (IAA), Epidermal Growth Factor (EGF), and FSH. To evaluate ultrastructure of primordial follicles cultured in MEM (control) or in MEM containing IAA, EGF, and FSH, fragments of cultured tissue were processes for transmission electron microscopy. Except in the control, primordial follicles cultured in supplemented media for 6¿d were ultrastructurally normal. They had oocyte with intact nucleus and the cytoplasm contained heterogeneous-sized lipid droplets and numerous round or elongated mitochondria with intact parallel cristae were observed. Rough endoplasmic reticulum (RER) was rarely found. The granulosa cells cytoplasm contained a great number of mitochondria and abundant RER. In conclusion, the presence of IAA, EGF, and FSH helped to maintain ultrastructural integrity of sheep primordial follicles cultured in vitro.",

author = "Andrade, {Evelyn Rabelo} and Poul Hyttel and Landim-Alvarenga, {Fernanda Da Cruz} and Silva, {Jos{\'e} Roberto Viana} and Alfieri, {Amauri Alcindo} and Seneda, {Marcelo Marcondes} and Figueiredo, {Jos{\'e} Ricardo} and Ricardo Toniolli",

year = "2011",

doi = "10.4061/2011/670987",

language = "English",

volume = "2011",

journal = "Veterinary Medicine International",

issn = "2042-0048",

publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - Ultrastructure of sheep primordial follicles cultured in the presence of indol acetic acid, EGF, and FSH

AU - Andrade, Evelyn Rabelo

AU - Hyttel, Poul

AU - Landim-Alvarenga, Fernanda Da Cruz

AU - Silva, José Roberto Viana

AU - Alfieri, Amauri Alcindo

AU - Seneda, Marcelo Marcondes

AU - Figueiredo, José Ricardo

AU - Toniolli, Ricardo

PY - 2011

Y1 - 2011

N2 - The aim of this study was to investigate the ultrastructural characteristics of primordial follicles after culturing of sheep ovarian cortical slices in the presence of indol acetic acid (IAA), Epidermal Growth Factor (EGF), and FSH. To evaluate ultrastructure of primordial follicles cultured in MEM (control) or in MEM containing IAA, EGF, and FSH, fragments of cultured tissue were processes for transmission electron microscopy. Except in the control, primordial follicles cultured in supplemented media for 6¿d were ultrastructurally normal. They had oocyte with intact nucleus and the cytoplasm contained heterogeneous-sized lipid droplets and numerous round or elongated mitochondria with intact parallel cristae were observed. Rough endoplasmic reticulum (RER) was rarely found. The granulosa cells cytoplasm contained a great number of mitochondria and abundant RER. In conclusion, the presence of IAA, EGF, and FSH helped to maintain ultrastructural integrity of sheep primordial follicles cultured in vitro.

AB - The aim of this study was to investigate the ultrastructural characteristics of primordial follicles after culturing of sheep ovarian cortical slices in the presence of indol acetic acid (IAA), Epidermal Growth Factor (EGF), and FSH. To evaluate ultrastructure of primordial follicles cultured in MEM (control) or in MEM containing IAA, EGF, and FSH, fragments of cultured tissue were processes for transmission electron microscopy. Except in the control, primordial follicles cultured in supplemented media for 6¿d were ultrastructurally normal. They had oocyte with intact nucleus and the cytoplasm contained heterogeneous-sized lipid droplets and numerous round or elongated mitochondria with intact parallel cristae were observed. Rough endoplasmic reticulum (RER) was rarely found. The granulosa cells cytoplasm contained a great number of mitochondria and abundant RER. In conclusion, the presence of IAA, EGF, and FSH helped to maintain ultrastructural integrity of sheep primordial follicles cultured in vitro.

U2 - 10.4061/2011/670987

DO - 10.4061/2011/670987

M3 - Journal article

SN - 2042-0048

VL - 2011

JO - Veterinary Medicine International

JF - Veterinary Medicine International

ER -