Abstract
The adoptive transfer of activated CD4+ alpha/beta T cell blasts from the spleens of immunocompetent C.B-17+/+ or BALB/cdm2 mice into C.B-17scid/scid (scid) mice induces a colitis in the scid recipient within 8 weeks, which progresses to severe disease within 16 weeks. T cells isolated from recipient colon show a Th1 cytokine phenotype. We have examined the relationship between the phenotype of the cellular infiltrate and the transcription and translation of the proinflammatory cytokine TNF-alpha. The techniques of double indirect immunohistology and in situ hybridization using digoxigenin-labelled riboprobes were used. The prominent myeloid cell infiltrate in diseased tissues comprised F4/80+, Mac-l+ macrophages, neutrophils, dendritic cells and activated macrophages. TNF-alpha transcription and translation were associated with activated macrophages in the lamina propria. Activated macrophages transcribing and translating TNF-alpha were clustered in areas of tissue destruction. Crypt epithelium of inflamed tissues transcribed TNF-alpha at a very early stage of the disease process, but translation of TNF-alpha protein could only be found in advanced epithelial dysplasia. This indicates differential post-transcriptional control of TNF-alpha in activated macrophages and the epithelium.
| Original language | English |
|---|---|
| Journal | Clinical and Experimental Immunology |
| Volume | 116 |
| Issue number | 3 |
| Pages (from-to) | 415-24 |
| Number of pages | 10 |
| ISSN | 0009-9104 |
| Publication status | Published - 1 Jun 1999 |
Keywords
- Adoptive Transfer
- Animals
- CD4-Positive T-Lymphocytes
- Colitis
- Disease Models, Animal
- Macrophage Activation
- Mice
- Mice, Inbred BALB C
- Mice, SCID
- Protein Biosynthesis
- RNA, Messenger
- Transcription, Genetic
- Tumor Necrosis Factor-alpha