Trichinella spiralis: Enteric mucin-related response to experimental infection in conventional and SPF pigs

TY - JOUR

T1 - Trichinella spiralis

T2 - Enteric mucin-related response to experimental infection in conventional and SPF pigs

AU - Theodoropoulos, G.

AU - Hicks, S. J.

AU - Corfield, A. P.

AU - Miller, B. G.

AU - Kapel, C. M O

AU - Trivizaki, M.

AU - Balaskas, C.

AU - Petrakos, G.

AU - Carrington, S. D.

PY - 2005/2

Y1 - 2005/2

N2 - Duodenal and jejunal responses to infection with Trichinella spiralis were compared in weaned piglets with a "normal dirty" vs. a "clean SPF" gut flora. Histochemical staining of neutral, acidic, sialylated, and sulphated residues was used to assess biosynthetic responses in mucin-secreting goblet cells. Peanut and Ulex lectins were also used to assess responses within the intestinal glycocalyx. Histomorphometric analysis was undertaken to evaluate the distribution and staining patterns of goblet cells in villi and crypts. Our analysis showed that stored mucin within goblet cells increased more in the infected conventional animals than in the infected SPF group. This was accompanied by changes in the pattern of sulphation and sialylation in the duodenum and jejunum. The thickness of the glycocalyx was increased in both duodenum and jejunum in both infected groups. However, this effect was greater for the infected SPF animals than the infected conventional animals. No significant differences were observed between uninfected conventional and uninfected SPF pigs.

AB - Duodenal and jejunal responses to infection with Trichinella spiralis were compared in weaned piglets with a "normal dirty" vs. a "clean SPF" gut flora. Histochemical staining of neutral, acidic, sialylated, and sulphated residues was used to assess biosynthetic responses in mucin-secreting goblet cells. Peanut and Ulex lectins were also used to assess responses within the intestinal glycocalyx. Histomorphometric analysis was undertaken to evaluate the distribution and staining patterns of goblet cells in villi and crypts. Our analysis showed that stored mucin within goblet cells increased more in the infected conventional animals than in the infected SPF group. This was accompanied by changes in the pattern of sulphation and sialylation in the duodenum and jejunum. The thickness of the glycocalyx was increased in both duodenum and jejunum in both infected groups. However, this effect was greater for the infected SPF animals than the infected conventional animals. No significant differences were observed between uninfected conventional and uninfected SPF pigs.

KW - Duodenum

KW - Histochemistry

KW - Histomorphometry

KW - Immunohistochemistry

KW - Jejunum

KW - Mucin

KW - Pig

KW - SPF

KW - Trichinella spiralis

UR - http://www.scopus.com/inward/record.url?scp=14744276919&partnerID=8YFLogxK

U2 - 10.1016/j.exppara.2004.11.006

DO - 10.1016/j.exppara.2004.11.006

M3 - Journal article

C2 - 15687012

AN - SCOPUS:14744276919

SN - 0014-4894

VL - 109

SP - 63

EP - 71

JO - Experimental Parasitology

JF - Experimental Parasitology

IS - 2

ER -