The impact of bioaugmentation on dechlorination kinetics and on microbial dechlorinating communities in subsurface clay till

Jacob Bælum; Charlotte Scheutz; Julie Claire Claudia Chambon; Christine Mosegaard Jensen; Rikke Prejh Brochmann; Philip Dennis; Troels Laier; Mette M Broholm; Poul L Bjerg; Philip John Binning; Carsten Suhr Jacobsen

doi:10.1016/j.envpol.2013.11.013

The impact of bioaugmentation on dechlorination kinetics and on microbial dechlorinating communities in subsurface clay till

Jacob Bælum, Charlotte Scheutz, Julie Claire Claudia Chambon, Christine Mosegaard Jensen, Rikke Prejh Brochmann, Philip Dennis, Troels Laier, Mette M Broholm, Poul L Bjerg, Philip John Binning, Carsten Suhr Jacobsen

8 Citations (Scopus)

Abstract

A molecular study on how the abundance of the dechlorinating culture KB-1 affects dechlorination rates in clay till is presented. DNA extracts showed changes in abundance of specific dechlorinators as well as their functional genes. Independently of the KB-1 added, the microbial dechlorinator abundance increased to the same level in all treatments. In the non-bioaugmented microcosms the reductive dehalogenase gene bvcA increased in abundance, but when KB-1 was added the related vcrA gene increased while bvcA genes did not increase. Modeling showed higher vinyl-chloride dechlorination rates and shorter time for complete dechlorination to ethene with higher initial concentration of KB-1 culture, while cis-dichloroethene dechlorination rates were not affected by KB-1 concentrations. This study provides high resolution abundance profiles of Dehalococcoides spp. (DHC) and functional genes, highlights the ecological behavior of KB-1 in clay till, and reinforces the importance of using multiple functional genes as biomarkers for reductive dechlorination.

Original language	English
Journal	Environmental Pollution
Volume	186
Pages (from-to)	149-157
Number of pages	9
ISSN	0269-7491
DOIs	https://doi.org/10.1016/j.envpol.2013.11.013
Publication status	Published - Mar 2014

Keywords

Aluminum Silicates
Biodegradation, Environmental
DNA, Bacterial
Ethylenes
Halogenation
Kinetics
Models, Chemical
Soil Microbiology
Soil Pollutants
Vinyl Chloride

Access to Document

10.1016/j.envpol.2013.11.013

Cite this

Bælum, J., Scheutz, C., Chambon, J. C. C., Jensen, C. M., Brochmann, R. P., Dennis, P., Laier, T., Broholm, M. M., Bjerg, P. L., Binning, P. J., & Jacobsen, C. S. (2014). The impact of bioaugmentation on dechlorination kinetics and on microbial dechlorinating communities in subsurface clay till. Environmental Pollution, 186, 149-157. https://doi.org/10.1016/j.envpol.2013.11.013

@article{40db01a121554154900a3193e05c7942,

title = "The impact of bioaugmentation on dechlorination kinetics and on microbial dechlorinating communities in subsurface clay till",

abstract = "A molecular study on how the abundance of the dechlorinating culture KB-1 affects dechlorination rates in clay till is presented. DNA extracts showed changes in abundance of specific dechlorinators as well as their functional genes. Independently of the KB-1 added, the microbial dechlorinator abundance increased to the same level in all treatments. In the non-bioaugmented microcosms the reductive dehalogenase gene bvcA increased in abundance, but when KB-1 was added the related vcrA gene increased while bvcA genes did not increase. Modeling showed higher vinyl-chloride dechlorination rates and shorter time for complete dechlorination to ethene with higher initial concentration of KB-1 culture, while cis-dichloroethene dechlorination rates were not affected by KB-1 concentrations. This study provides high resolution abundance profiles of Dehalococcoides spp. (DHC) and functional genes, highlights the ecological behavior of KB-1 in clay till, and reinforces the importance of using multiple functional genes as biomarkers for reductive dechlorination.",

keywords = "Aluminum Silicates, Biodegradation, Environmental, DNA, Bacterial, Ethylenes, Halogenation, Kinetics, Models, Chemical, Soil Microbiology, Soil Pollutants, Vinyl Chloride",

author = "Jacob B{\ae}lum and Charlotte Scheutz and Chambon, {Julie Claire Claudia} and Jensen, {Christine Mosegaard} and Brochmann, {Rikke Prejh} and Philip Dennis and Troels Laier and Broholm, {Mette M} and Bjerg, {Poul L} and Binning, {Philip John} and Jacobsen, {Carsten Suhr}",

year = "2014",

month = mar,

doi = "10.1016/j.envpol.2013.11.013",

language = "English",

volume = "186",

pages = "149--157",

journal = "Environmental Pollution",

issn = "0269-7491",

publisher = "Pergamon Press",

}

TY - JOUR

T1 - The impact of bioaugmentation on dechlorination kinetics and on microbial dechlorinating communities in subsurface clay till

AU - Bælum, Jacob

AU - Scheutz, Charlotte

AU - Chambon, Julie Claire Claudia

AU - Jensen, Christine Mosegaard

AU - Brochmann, Rikke Prejh

AU - Dennis, Philip

AU - Laier, Troels

AU - Broholm, Mette M

AU - Bjerg, Poul L

AU - Binning, Philip John

AU - Jacobsen, Carsten Suhr

PY - 2014/3

Y1 - 2014/3

N2 - A molecular study on how the abundance of the dechlorinating culture KB-1 affects dechlorination rates in clay till is presented. DNA extracts showed changes in abundance of specific dechlorinators as well as their functional genes. Independently of the KB-1 added, the microbial dechlorinator abundance increased to the same level in all treatments. In the non-bioaugmented microcosms the reductive dehalogenase gene bvcA increased in abundance, but when KB-1 was added the related vcrA gene increased while bvcA genes did not increase. Modeling showed higher vinyl-chloride dechlorination rates and shorter time for complete dechlorination to ethene with higher initial concentration of KB-1 culture, while cis-dichloroethene dechlorination rates were not affected by KB-1 concentrations. This study provides high resolution abundance profiles of Dehalococcoides spp. (DHC) and functional genes, highlights the ecological behavior of KB-1 in clay till, and reinforces the importance of using multiple functional genes as biomarkers for reductive dechlorination.

AB - A molecular study on how the abundance of the dechlorinating culture KB-1 affects dechlorination rates in clay till is presented. DNA extracts showed changes in abundance of specific dechlorinators as well as their functional genes. Independently of the KB-1 added, the microbial dechlorinator abundance increased to the same level in all treatments. In the non-bioaugmented microcosms the reductive dehalogenase gene bvcA increased in abundance, but when KB-1 was added the related vcrA gene increased while bvcA genes did not increase. Modeling showed higher vinyl-chloride dechlorination rates and shorter time for complete dechlorination to ethene with higher initial concentration of KB-1 culture, while cis-dichloroethene dechlorination rates were not affected by KB-1 concentrations. This study provides high resolution abundance profiles of Dehalococcoides spp. (DHC) and functional genes, highlights the ecological behavior of KB-1 in clay till, and reinforces the importance of using multiple functional genes as biomarkers for reductive dechlorination.

KW - Aluminum Silicates

KW - Biodegradation, Environmental

KW - DNA, Bacterial

KW - Ethylenes

KW - Halogenation

KW - Kinetics

KW - Models, Chemical

KW - Soil Microbiology

KW - Soil Pollutants

KW - Vinyl Chloride

U2 - 10.1016/j.envpol.2013.11.013

DO - 10.1016/j.envpol.2013.11.013

M3 - Journal article

C2 - 24374375

SN - 0269-7491

VL - 186

SP - 149

EP - 157

JO - Environmental Pollution

JF - Environmental Pollution

ER -

The impact of bioaugmentation on dechlorination kinetics and on microbial dechlorinating communities in subsurface clay till

Abstract

Keywords

Access to Document

Fingerprint

Cite this