The CC-chemokine receptor 5 (CCR5) is a marker of, but not essential for the development of human Th1 cells

TY - JOUR

T1 - The CC-chemokine receptor 5 (CCR5) is a marker of, but not essential for the development of human Th1 cells

AU - Odum, Niels

AU - Bregenholt, S

AU - Eriksen, K W

AU - Skov, S

AU - Ryder, Lars P.

AU - Bendtzen, Klaus

AU - Van Neerven, R J

AU - Svejgaard, A

AU - Garred, P

N1 - Keywords: Biological Markers; Cell Differentiation; Cell Lineage; Cells, Cultured; Flow Cytometry; Humans; Receptors, CCR5; Th1 Cells

PY - 1999

Y1 - 1999

N2 - The CC-chemokine receptor 5 (CCR5) has recently been described as a surface marker of human T cells producing type 1 (Th1) cytokines. Here we confirm that CCR5 is expressed on human Th1 but not on Th2 T-cell clones. Using intracellular cytokine staining, we show that alloantigen specific CD4+ T-cell lines derived from a CCR5-deficient individual (delta32 allele homozygote) contain high numbers of both interferon gamma (IFN-gamma) and interleukin (IL)-2 producing cells, low numbers of IL-10 producing cells and no IL4 or IL-5 producing cells when stimulated with phorbol ester and ionomycin in vitro. These results were similar to those obtained from alloantigen specific CD4+ T-cell lines derived from CCR5 expressing individuals. An enzyme-linked immunoabsorbent assay (ELISA) confirmed that the Th1 cytokine-positive cells from the CCR5-deficient individual were able to produce equal amounts of cytokines when compared to T-cell lines from CCR5-expressing individuals, These results demonstrate that CCR5-negative T cells display the same capacity of Th1 T-cell differentiation as T cells derived from CCR5-expressing individuals. Thus, CCR5 expression is not essential for differentiation of human Th1 T cells.

AB - The CC-chemokine receptor 5 (CCR5) has recently been described as a surface marker of human T cells producing type 1 (Th1) cytokines. Here we confirm that CCR5 is expressed on human Th1 but not on Th2 T-cell clones. Using intracellular cytokine staining, we show that alloantigen specific CD4+ T-cell lines derived from a CCR5-deficient individual (delta32 allele homozygote) contain high numbers of both interferon gamma (IFN-gamma) and interleukin (IL)-2 producing cells, low numbers of IL-10 producing cells and no IL4 or IL-5 producing cells when stimulated with phorbol ester and ionomycin in vitro. These results were similar to those obtained from alloantigen specific CD4+ T-cell lines derived from CCR5 expressing individuals. An enzyme-linked immunoabsorbent assay (ELISA) confirmed that the Th1 cytokine-positive cells from the CCR5-deficient individual were able to produce equal amounts of cytokines when compared to T-cell lines from CCR5-expressing individuals, These results demonstrate that CCR5-negative T cells display the same capacity of Th1 T-cell differentiation as T cells derived from CCR5-expressing individuals. Thus, CCR5 expression is not essential for differentiation of human Th1 T cells.

U2 - 10.1034/j.1399-0039.1999.540606.x

DO - 10.1034/j.1399-0039.1999.540606.x

M3 - Journal article

C2 - 10674971

SN - 2059-2302

VL - 54

SP - 572

EP - 577

JO - HLA

JF - HLA

IS - 6

ER -