@inbook{f4560f7ee4e64ef4a3c2fdc04cd33889,
title = "Synthesis of O-glycopeptides and construction of glycopeptide microarrays",
abstract = "O-glycosylation of proteins is an important modification which affects biological function and immunity. In this chapter, we provide protocols for efficient solid-phase O-glycopeptide synthesis (SPGPS) and protocols for the construction of glycopeptide microarray chips for screening applications. This will be exemplified for mucin-type glycopeptides and the construction of glycopeptide microarrays. To this end, the protocols provided are particularly suited for small-scale robotic parallel synthesis. N-Terminal amine capping of deletion peptides during synthesis stands out as vital to this strategy. It allows for direct on-slide enrichment of the full-length target product and thereby bypasses tedious isolation and purification procedures.",
keywords = "Amino Acids, Fluorenes, Glycopeptides, Polysaccharides, Protein Array Analysis, Solid-Phase Synthesis Techniques",
author = "Blixt, {Klas Ola} and Emiliano Cl{\'o}",
year = "2013",
doi = "10.1007/978-1-62703-544-6_14",
language = "English",
isbn = "978-1-62703-543-9",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "201--214",
editor = "Jensen, {Knud J.} and Shelton, {Pernille Tofteng} and Pedersen, {S{\o}ren L.}",
booktitle = "Peptide synthesis and applications",
address = "United States",
}