Studying RNA-protein interactions in vivo by RNA immunoprecipitation

TY - CHAP

T1 - Studying RNA-protein interactions in vivo by RNA immunoprecipitation

AU - Selth, Luke A

AU - Close, Pierre

AU - Svejstrup, Jesper Q

PY - 2011

Y1 - 2011

N2 - The crucial roles played by RNA-binding proteins in all aspects of RNA metabolism, particularly in the regulation of transcription, have become increasingly evident. Moreover, other factors that do not directly interact with RNA molecules can nevertheless function proximally to RNA polymerases and have significant effects on gene expression. RNA immunoprecipitation (RIP) is a powerful technique used to detect direct and indirect interactions between individual proteins and specific RNA molecules in vivo. Here, we describe RIP methods for both yeast and mammalian cells.

AB - The crucial roles played by RNA-binding proteins in all aspects of RNA metabolism, particularly in the regulation of transcription, have become increasingly evident. Moreover, other factors that do not directly interact with RNA molecules can nevertheless function proximally to RNA polymerases and have significant effects on gene expression. RNA immunoprecipitation (RIP) is a powerful technique used to detect direct and indirect interactions between individual proteins and specific RNA molecules in vivo. Here, we describe RIP methods for both yeast and mammalian cells.

KW - Chromatin

KW - Fungal Proteins

KW - HEK293 Cells

KW - Humans

KW - Immunoprecipitation

KW - Protein Binding

KW - RNA, Fungal

KW - Saccharomyces cerevisiae

U2 - 10.1007/978-1-61779-316-5_19

DO - 10.1007/978-1-61779-316-5_19

M3 - Book chapter

C2 - 21913085

VL - 791

SP - 253

EP - 264

BT - Methods in Molecular Biology

PB - Springer Science+Business Media

ER -