Structure of a SARS coronavirus-derived peptide bound to the human major histocompatibility complex class I molecule HLA-B*1501

Gustav Røder; Ole Kristensen; Jette S Kastrup; Søren Buus; Michael Gajhede

doi:10.1107/S1744309108012396

Structure of a SARS coronavirus-derived peptide bound to the human major histocompatibility complex class I molecule HLA-B*1501

Gustav Røder, Ole Kristensen, Jette S Kastrup, Søren Buus, Michael Gajhede

Department of Immunology and Microbiology

6 Citations (Scopus)

Abstract

The human leukocyte antigen (HLA) class I system comprises a highly polymorphic set of molecules that specifically bind and present peptides to cytotoxic T cells. HLA-B*1501 is a prototypical member of the HLA-B62 supertype and only two peptide-HLA-B*1501 structures have been determined. Here, the crystal structure of HLA-B*1501 in complex with a SARS coronavirus-derived nonapeptide (VQQESSFVM) has been determined at high resolution (1.87 A). The peptide is deeply anchored in the B and F pockets, but with the Glu4 residue pointing away from the floor in the peptide-binding groove, making it available for interactions with a potential T-cell receptor.

Original language	English
Journal	Acta Crystallographica. Section F : Structural Biology and Crystallization Communications
Volume	64
Issue number	Pt 6
Pages (from-to)	459-62
Number of pages	3
ISSN	1744-3091
DOIs	https://doi.org/10.1107/S1744309108012396
Publication status	Published - 2008

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Structure of a SARS coronavirus-derived peptide bound to the human major histocompatibility complex class I molecule HLA-B*1501. / Røder, Gustav; Kristensen, Ole; Kastrup, Jette S et al.

In: Acta Crystallographica. Section F : Structural Biology and Crystallization Communications, Vol. 64, No. Pt 6, 2008, p. 459-62.

Research output: Contribution to journal › Journal article › Research › peer-review

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abstract = "The human leukocyte antigen (HLA) class I system comprises a highly polymorphic set of molecules that specifically bind and present peptides to cytotoxic T cells. HLA-B*1501 is a prototypical member of the HLA-B62 supertype and only two peptide-HLA-B*1501 structures have been determined. Here, the crystal structure of HLA-B*1501 in complex with a SARS coronavirus-derived nonapeptide (VQQESSFVM) has been determined at high resolution (1.87 A). The peptide is deeply anchored in the B and F pockets, but with the Glu4 residue pointing away from the floor in the peptide-binding groove, making it available for interactions with a potential T-cell receptor.",

author = "Gustav R{\o}der and Ole Kristensen and Kastrup, {Jette S} and S{\o}ren Buus and Michael Gajhede",

note = "Keywords: Binding Sites; Crystallography, X-Ray; HLA-B Antigens; Histocompatibility Antigens Class I; Humans; Hydrogen Bonding; Hydrophobicity; Ligands; Models, Molecular; Mutagenesis, Site-Directed; Oligopeptides; Protein Binding; Protein Structure, Secondary; SARS Virus; Water",

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AU - Røder, Gustav

AU - Kristensen, Ole

AU - Kastrup, Jette S

AU - Buus, Søren

AU - Gajhede, Michael

N1 - Keywords: Binding Sites; Crystallography, X-Ray; HLA-B Antigens; Histocompatibility Antigens Class I; Humans; Hydrogen Bonding; Hydrophobicity; Ligands; Models, Molecular; Mutagenesis, Site-Directed; Oligopeptides; Protein Binding; Protein Structure, Secondary; SARS Virus; Water

PY - 2008

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N2 - The human leukocyte antigen (HLA) class I system comprises a highly polymorphic set of molecules that specifically bind and present peptides to cytotoxic T cells. HLA-B*1501 is a prototypical member of the HLA-B62 supertype and only two peptide-HLA-B*1501 structures have been determined. Here, the crystal structure of HLA-B*1501 in complex with a SARS coronavirus-derived nonapeptide (VQQESSFVM) has been determined at high resolution (1.87 A). The peptide is deeply anchored in the B and F pockets, but with the Glu4 residue pointing away from the floor in the peptide-binding groove, making it available for interactions with a potential T-cell receptor.

AB - The human leukocyte antigen (HLA) class I system comprises a highly polymorphic set of molecules that specifically bind and present peptides to cytotoxic T cells. HLA-B*1501 is a prototypical member of the HLA-B62 supertype and only two peptide-HLA-B*1501 structures have been determined. Here, the crystal structure of HLA-B*1501 in complex with a SARS coronavirus-derived nonapeptide (VQQESSFVM) has been determined at high resolution (1.87 A). The peptide is deeply anchored in the B and F pockets, but with the Glu4 residue pointing away from the floor in the peptide-binding groove, making it available for interactions with a potential T-cell receptor.

U2 - 10.1107/S1744309108012396

DO - 10.1107/S1744309108012396

M3 - Journal article

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SN - 2053-230X

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JO - Acta Crystallographica Section F: Structural Biology Communications

JF - Acta Crystallographica Section F: Structural Biology Communications

IS - Pt 6

ER -

Structure of a SARS coronavirus-derived peptide bound to the human major histocompatibility complex class I molecule HLA-B*1501

Abstract

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