Staurosporine-induced cell death in Tetrahymena thermophila has mixed characteristics of both apoptotic and autophagic degeneration

S T Christensen; J Chemnitz; E M Straarup; Karsten Kristiansen; D N Wheatley; L Rasmussen

doi:10.1006/cbir.1998.0320

Staurosporine-induced cell death in Tetrahymena thermophila has mixed characteristics of both apoptotic and autophagic degeneration

S T Christensen, J Chemnitz, E M Straarup, Karsten Kristiansen, D N Wheatley, L Rasmussen

Department of Biology

59 Citations (Scopus)

Abstract

Staurosporine blocks signal transduction associated with cell survival, proliferation and chemosensory behaviour in the ciliated protozoan, Tetrahymena thermophila. Staurosporine inhibits cell proliferation and in vivo protein phosphorylation induced by phorbol ester. It also reduces the in vitro phosphorylation of the PKC-specific substrate, myelin basic protein fragment 4-14. Our results show that cell death in the presence of staurosporine is associated with morphological and ultrastructural changes similar to both apoptosis and autophagic degeneration, but these in turn can be postponed or prevented by 8-bromo-cyclic GMP, protoporphyrin IX, hemin or actinomycin D, although phorbol ester and insulin were ineffective. The results support the notion that staurosporine-induced cell death is an active process, associated with and/or requiring de novo RNA synthesis.

Original language	English
Journal	Cell Biology International
Volume	22
Issue number	7-8
Pages (from-to)	591-598
Number of pages	7
ISSN	1065-6995
DOIs	https://doi.org/10.1006/cbir.1998.0320
Publication status	Published - 1998

Access to Document

10.1006/cbir.1998.0320

Cite this

@article{1edece3074c111dbbee902004c4f4f50,

title = "Staurosporine-induced cell death in Tetrahymena thermophila has mixed characteristics of both apoptotic and autophagic degeneration",

abstract = "Staurosporine blocks signal transduction associated with cell survival, proliferation and chemosensory behaviour in the ciliated protozoan, Tetrahymena thermophila. Staurosporine inhibits cell proliferation and in vivo protein phosphorylation induced by phorbol ester. It also reduces the in vitro phosphorylation of the PKC-specific substrate, myelin basic protein fragment 4-14. Our results show that cell death in the presence of staurosporine is associated with morphological and ultrastructural changes similar to both apoptosis and autophagic degeneration, but these in turn can be postponed or prevented by 8-bromo-cyclic GMP, protoporphyrin IX, hemin or actinomycin D, although phorbol ester and insulin were ineffective. The results support the notion that staurosporine-induced cell death is an active process, associated with and/or requiring de novo RNA synthesis.",

author = "Christensen, {S T} and J Chemnitz and Straarup, {E M} and Karsten Kristiansen and Wheatley, {D N} and L Rasmussen",

note = "Keywords: Animals; Apoptosis; Autophagy; Enzyme Inhibitors; Staurosporine; Tetrahymena thermophila",

year = "1998",

doi = "10.1006/cbir.1998.0320",

language = "English",

volume = "22",

pages = "591--598",

journal = "Cell Biology International",

issn = "1065-6995",

publisher = "Academic Press",

number = "7-8",

}

TY - JOUR

T1 - Staurosporine-induced cell death in Tetrahymena thermophila has mixed characteristics of both apoptotic and autophagic degeneration

AU - Christensen, S T

AU - Chemnitz, J

AU - Straarup, E M

AU - Kristiansen, Karsten

AU - Wheatley, D N

AU - Rasmussen, L

N1 - Keywords: Animals; Apoptosis; Autophagy; Enzyme Inhibitors; Staurosporine; Tetrahymena thermophila

PY - 1998

Y1 - 1998

N2 - Staurosporine blocks signal transduction associated with cell survival, proliferation and chemosensory behaviour in the ciliated protozoan, Tetrahymena thermophila. Staurosporine inhibits cell proliferation and in vivo protein phosphorylation induced by phorbol ester. It also reduces the in vitro phosphorylation of the PKC-specific substrate, myelin basic protein fragment 4-14. Our results show that cell death in the presence of staurosporine is associated with morphological and ultrastructural changes similar to both apoptosis and autophagic degeneration, but these in turn can be postponed or prevented by 8-bromo-cyclic GMP, protoporphyrin IX, hemin or actinomycin D, although phorbol ester and insulin were ineffective. The results support the notion that staurosporine-induced cell death is an active process, associated with and/or requiring de novo RNA synthesis.

AB - Staurosporine blocks signal transduction associated with cell survival, proliferation and chemosensory behaviour in the ciliated protozoan, Tetrahymena thermophila. Staurosporine inhibits cell proliferation and in vivo protein phosphorylation induced by phorbol ester. It also reduces the in vitro phosphorylation of the PKC-specific substrate, myelin basic protein fragment 4-14. Our results show that cell death in the presence of staurosporine is associated with morphological and ultrastructural changes similar to both apoptosis and autophagic degeneration, but these in turn can be postponed or prevented by 8-bromo-cyclic GMP, protoporphyrin IX, hemin or actinomycin D, although phorbol ester and insulin were ineffective. The results support the notion that staurosporine-induced cell death is an active process, associated with and/or requiring de novo RNA synthesis.

U2 - 10.1006/cbir.1998.0320

DO - 10.1006/cbir.1998.0320

M3 - Journal article

C2 - 10452827

SN - 1065-6995

VL - 22

SP - 591

EP - 598

JO - Cell Biology International

JF - Cell Biology International

IS - 7-8

ER -

Staurosporine-induced cell death in Tetrahymena thermophila has mixed characteristics of both apoptotic and autophagic degeneration

Abstract

Access to Document

Fingerprint

Cite this