Spontaneous human squamous cell carcinomas are killed by a human cytotoxic T lymphocyte clone recognizing a wild-type p53-derived peptide

M Röpke; J Hald; Per Guldberg; J Zeuthen; L Nørgaard; L Fugger; A Svejgaard; S Van der Burg; H W Nijman; C J Melief; Mogens Helweg Claesson

Spontaneous human squamous cell carcinomas are killed by a human cytotoxic T lymphocyte clone recognizing a wild-type p53-derived peptide

M Röpke, J Hald, Per Guldberg, J Zeuthen, L Nørgaard, L Fugger, A Svejgaard, S Van der Burg, H W Nijman, C J Melief, Mogens Helweg Claesson

Department of Immunology and Microbiology

145 Citations (Scopus)

Abstract

A cytotoxic T lymphocyte (CTL) clone generated in vitro from the peripheral blood of a healthy HLA-A2-positive individual against a synthetic p53 protein-derived wild-type peptide (L9V) was shown to kill squamous carcinoma cell lines derived from two head and neck carcinomas, which expressed mutant p53 genes, in a L9V/HLA-A2 specific and restricted fashion. Thus, the normal tolerance against endogenously processed p53 protein-derived self-epitopes can be broken by peptide-specific in vitro priming. p53 protein-derived wild-type peptides might thus represent tumor associated target molecules for immunotherapeutical approaches.

Original language	English
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	93
Issue number	25
Pages (from-to)	14704-7
Number of pages	4
ISSN	0027-8424
Publication status	Published - 10 Dec 1996

Keywords

Carcinoma, Squamous Cell
Cytotoxicity, Immunologic
HLA-A2 Antigen
Head and Neck Neoplasms
Humans
Mutation
T-Lymphocytes, Cytotoxic
Tumor Cells, Cultured
Tumor Suppressor Protein p53

Cite this

Röpke, M., Hald, J., Guldberg, P., Zeuthen, J., Nørgaard, L., Fugger, L., Svejgaard, A., Van der Burg, S., Nijman, H. W., Melief, C. J., & Claesson, M. H. (1996). Spontaneous human squamous cell carcinomas are killed by a human cytotoxic T lymphocyte clone recognizing a wild-type p53-derived peptide. Proceedings of the National Academy of Sciences of the United States of America, 93(25), 14704-7.

Spontaneous human squamous cell carcinomas are killed by a human cytotoxic T lymphocyte clone recognizing a wild-type p53-derived peptide. / Röpke, M; Hald, J; Guldberg, Per et al.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 25, 10.12.1996, p. 14704-7.

Research output: Contribution to journal › Journal article › Research › peer-review

Röpke, M, Hald, J, Guldberg, P, Zeuthen, J, Nørgaard, L, Fugger, L, Svejgaard, A, Van der Burg, S, Nijman, HW, Melief, CJ & Claesson, MH 1996, 'Spontaneous human squamous cell carcinomas are killed by a human cytotoxic T lymphocyte clone recognizing a wild-type p53-derived peptide', Proceedings of the National Academy of Sciences of the United States of America, vol. 93, no. 25, pp. 14704-7.

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title = "Spontaneous human squamous cell carcinomas are killed by a human cytotoxic T lymphocyte clone recognizing a wild-type p53-derived peptide",

abstract = "A cytotoxic T lymphocyte (CTL) clone generated in vitro from the peripheral blood of a healthy HLA-A2-positive individual against a synthetic p53 protein-derived wild-type peptide (L9V) was shown to kill squamous carcinoma cell lines derived from two head and neck carcinomas, which expressed mutant p53 genes, in a L9V/HLA-A2 specific and restricted fashion. Thus, the normal tolerance against endogenously processed p53 protein-derived self-epitopes can be broken by peptide-specific in vitro priming. p53 protein-derived wild-type peptides might thus represent tumor associated target molecules for immunotherapeutical approaches.",

keywords = "Carcinoma, Squamous Cell, Cytotoxicity, Immunologic, HLA-A2 Antigen, Head and Neck Neoplasms, Humans, Mutation, T-Lymphocytes, Cytotoxic, Tumor Cells, Cultured, Tumor Suppressor Protein p53",

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T1 - Spontaneous human squamous cell carcinomas are killed by a human cytotoxic T lymphocyte clone recognizing a wild-type p53-derived peptide

AU - Röpke, M

AU - Hald, J

AU - Guldberg, Per

AU - Zeuthen, J

AU - Nørgaard, L

AU - Fugger, L

AU - Svejgaard, A

AU - Van der Burg, S

AU - Nijman, H W

AU - Melief, C J

AU - Claesson, Mogens Helweg

PY - 1996/12/10

Y1 - 1996/12/10

N2 - A cytotoxic T lymphocyte (CTL) clone generated in vitro from the peripheral blood of a healthy HLA-A2-positive individual against a synthetic p53 protein-derived wild-type peptide (L9V) was shown to kill squamous carcinoma cell lines derived from two head and neck carcinomas, which expressed mutant p53 genes, in a L9V/HLA-A2 specific and restricted fashion. Thus, the normal tolerance against endogenously processed p53 protein-derived self-epitopes can be broken by peptide-specific in vitro priming. p53 protein-derived wild-type peptides might thus represent tumor associated target molecules for immunotherapeutical approaches.

AB - A cytotoxic T lymphocyte (CTL) clone generated in vitro from the peripheral blood of a healthy HLA-A2-positive individual against a synthetic p53 protein-derived wild-type peptide (L9V) was shown to kill squamous carcinoma cell lines derived from two head and neck carcinomas, which expressed mutant p53 genes, in a L9V/HLA-A2 specific and restricted fashion. Thus, the normal tolerance against endogenously processed p53 protein-derived self-epitopes can be broken by peptide-specific in vitro priming. p53 protein-derived wild-type peptides might thus represent tumor associated target molecules for immunotherapeutical approaches.

KW - Carcinoma, Squamous Cell

KW - Cytotoxicity, Immunologic

KW - HLA-A2 Antigen

KW - Head and Neck Neoplasms

KW - Humans

KW - Mutation

KW - T-Lymphocytes, Cytotoxic

KW - Tumor Cells, Cultured

KW - Tumor Suppressor Protein p53

M3 - Journal article

C2 - 8962118

SN - 0027-8424

VL - 93

SP - 14704

EP - 14707

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 25

ER -

Spontaneous human squamous cell carcinomas are killed by a human cytotoxic T lymphocyte clone recognizing a wild-type p53-derived peptide

Abstract

Keywords

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