Sperm DNA integrity in relation to exposure to environmental perfluoroalkyl substances - a study of spouses of pregnant women in three geographical regions

Ina Olmer Specht; Karin S Hougaard; Marcello Spanò; Davide Bizzaro; Gian Carlo Manicardi; Christian H Lindh; Gunnar Toft; Bo A G Jönsson; Aleksander Giwercman; Jens Peter E Bonde

doi:10.1016/j.reprotox.2012.02.008

Sperm DNA integrity in relation to exposure to environmental perfluoroalkyl substances - a study of spouses of pregnant women in three geographical regions

Ina Olmer Specht, Karin S Hougaard, Marcello Spanò, Davide Bizzaro, Gian Carlo Manicardi, Christian H Lindh, Gunnar Toft, Bo A G Jönsson, Aleksander Giwercman, Jens Peter E Bonde

48 Citations (Scopus)

Abstract

Perfluoroalkyl substances (PFASs) can interfere with male reproductive function, but evidence in humans is limited. Six hundred four fertile men (199 from Greenland, 197 from Poland and 208 from Ukraine) were enrolled in the study. We measured four PFASs in serum (PFOS, PFOA, PFNA and PFHxS) and concurrent DNA damage in spermatozoa by sperm chromatin structure assay (SCSA) and in situ terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay, apoptotic markers in semen (Fas-receptor and Bcl-xL), and reproductive hormones in serum. No association between PFASs and SCSA, apoptotic markers or reproductive hormones emerged. We observed a slight increase in SHBG and TUNEL-positivity with increased PFOA exposure in men from Greenland. Thus, consistent evidence that PFAS exposure interferes with sperm DNA fragmentation, apoptosis or reproductive hormones was not found.

Original language	English
Journal	Reproductive Toxicology
Volume	33
Issue number	4
Pages (from-to)	577-83
Number of pages	7
ISSN	0890-6238
DOIs	https://doi.org/10.1016/j.reprotox.2012.02.008
Publication status	Published - Jul 2012

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Specht, I. O., Hougaard, K. S., Spanò, M., Bizzaro, D., Manicardi, G. C., Lindh, C. H., Toft, G., Jönsson, B. A. G., Giwercman, A., & Bonde, J. P. E. (2012). Sperm DNA integrity in relation to exposure to environmental perfluoroalkyl substances - a study of spouses of pregnant women in three geographical regions. Reproductive Toxicology, 33(4), 577-83. https://doi.org/10.1016/j.reprotox.2012.02.008

Sperm DNA integrity in relation to exposure to environmental perfluoroalkyl substances - a study of spouses of pregnant women in three geographical regions. / Specht, Ina Olmer; Hougaard, Karin S; Spanò, Marcello et al.

In: Reproductive Toxicology, Vol. 33, No. 4, 07.2012, p. 577-83.

Research output: Contribution to journal › Journal article › Research › peer-review

Specht, IO, Hougaard, KS, Spanò, M, Bizzaro, D, Manicardi, GC, Lindh, CH, Toft, G, Jönsson, BAG, Giwercman, A & Bonde, JPE 2012, 'Sperm DNA integrity in relation to exposure to environmental perfluoroalkyl substances - a study of spouses of pregnant women in three geographical regions', Reproductive Toxicology, vol. 33, no. 4, pp. 577-83. https://doi.org/10.1016/j.reprotox.2012.02.008

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title = "Sperm DNA integrity in relation to exposure to environmental perfluoroalkyl substances - a study of spouses of pregnant women in three geographical regions",

abstract = "Perfluoroalkyl substances (PFASs) can interfere with male reproductive function, but evidence in humans is limited. Six hundred four fertile men (199 from Greenland, 197 from Poland and 208 from Ukraine) were enrolled in the study. We measured four PFASs in serum (PFOS, PFOA, PFNA and PFHxS) and concurrent DNA damage in spermatozoa by sperm chromatin structure assay (SCSA) and in situ terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay, apoptotic markers in semen (Fas-receptor and Bcl-xL), and reproductive hormones in serum. No association between PFASs and SCSA, apoptotic markers or reproductive hormones emerged. We observed a slight increase in SHBG and TUNEL-positivity with increased PFOA exposure in men from Greenland. Thus, consistent evidence that PFAS exposure interferes with sperm DNA fragmentation, apoptosis or reproductive hormones was not found.",

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T1 - Sperm DNA integrity in relation to exposure to environmental perfluoroalkyl substances - a study of spouses of pregnant women in three geographical regions

AU - Specht, Ina Olmer

AU - Hougaard, Karin S

AU - Spanò, Marcello

AU - Bizzaro, Davide

AU - Manicardi, Gian Carlo

AU - Lindh, Christian H

AU - Toft, Gunnar

AU - Jönsson, Bo A G

AU - Giwercman, Aleksander

AU - Bonde, Jens Peter E

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N2 - Perfluoroalkyl substances (PFASs) can interfere with male reproductive function, but evidence in humans is limited. Six hundred four fertile men (199 from Greenland, 197 from Poland and 208 from Ukraine) were enrolled in the study. We measured four PFASs in serum (PFOS, PFOA, PFNA and PFHxS) and concurrent DNA damage in spermatozoa by sperm chromatin structure assay (SCSA) and in situ terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay, apoptotic markers in semen (Fas-receptor and Bcl-xL), and reproductive hormones in serum. No association between PFASs and SCSA, apoptotic markers or reproductive hormones emerged. We observed a slight increase in SHBG and TUNEL-positivity with increased PFOA exposure in men from Greenland. Thus, consistent evidence that PFAS exposure interferes with sperm DNA fragmentation, apoptosis or reproductive hormones was not found.

AB - Perfluoroalkyl substances (PFASs) can interfere with male reproductive function, but evidence in humans is limited. Six hundred four fertile men (199 from Greenland, 197 from Poland and 208 from Ukraine) were enrolled in the study. We measured four PFASs in serum (PFOS, PFOA, PFNA and PFHxS) and concurrent DNA damage in spermatozoa by sperm chromatin structure assay (SCSA) and in situ terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay, apoptotic markers in semen (Fas-receptor and Bcl-xL), and reproductive hormones in serum. No association between PFASs and SCSA, apoptotic markers or reproductive hormones emerged. We observed a slight increase in SHBG and TUNEL-positivity with increased PFOA exposure in men from Greenland. Thus, consistent evidence that PFAS exposure interferes with sperm DNA fragmentation, apoptosis or reproductive hormones was not found.

U2 - 10.1016/j.reprotox.2012.02.008

DO - 10.1016/j.reprotox.2012.02.008

M3 - Journal article

C2 - 22449571

SN - 0890-6238

VL - 33

SP - 577

EP - 583

JO - Reproductive Toxicology

JF - Reproductive Toxicology

IS - 4

ER -

Sperm DNA integrity in relation to exposure to environmental perfluoroalkyl substances - a study of spouses of pregnant women in three geographical regions

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