@inbook{1cf41936bc8b417681e9486eaa351648,
title = "Solid-phase synthesis of phosphopeptides",
abstract = "Phosphopeptides are generally prepared by incorporation of suitable, protected phosphoamino acid derivatives during peptide synthesis using routine coupling protocols. The feasibility of chemical synthesis of phosphorylated peptides by Fmoc-SPPS was greatly enhanced by the introduction of the monobenzyl protecting group for the phosphate group. This minimized β-elimination of the phosphate group and made Fmoc-based synthesis of phosphopeptides the preferred synthesis strategy. Described here is our strategy for the synthesis of phosphopeptides attached to the solid support PEGA via a backbone amide linker type. This linker allows removal of side-chain protection groups without releasing the phosphopeptide from the solid support, thus enabling solid-phase-based pull-down reactions and peptide-protein interaction studies.",
keywords = "Backbone amide linker, Handle, Peptide, Aldehyde, Thioester, N-Alkyl amide, Cyclic peptide, Reductive amination",
author = "H{\o}jlys-Larsen, {Kim B.} and Jensen, {Knud J{\o}rgen}",
year = "2013",
doi = "10.1007/978-1-62703-544-6_13",
language = "English",
isbn = "978-1-62703-543-9",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "191--199",
editor = "Jensen, {Knud J.} and Shelton, {Pernille T.} and Pedersen, {S{\o}ren L.}",
booktitle = "Peptide synthesis and applications",
address = "United States",
}