Abstract
To elucidate the role of luminal nutrients and glucagon-like peptide-2 (GLP-2) in intestinal adaptation, rats were subjected to 70% midjejunoileal resection or ileal transection and were maintained with total parenteral nutrition (TPN) or oral feeding. TPN rats showed small bowel mucosal hyperplasia at 8 h through 7 days after resection, demonstrating that exogenous luminal nutrients are not essential for resection-induced adaptation when residual ileum and colon are present. Increased enterocyte proliferation was a stronger determinant of resection-induced mucosal growth in orally fed animals, whereas decreased apoptosis showed a greater effect in TPN animals. Resection induced significant transient increases in plasma bioactive GLP-2 during TPN, whereas resection induced sustained increases in plasma GLP-2 during oral feeding. Resection-induced adaptive growth in TPN and orally fed rats was associated with a significant positive correlation between increases in plasma bioactive GLP-2 and proglucagon mRNA expression in the colon of TPN rats and ileum of orally fed rats. These data support a significant role for endogenous GLP-2 in the adaptive response to mid-small bowel resection in both TPN and orally fed rats.
| Original language | English |
|---|---|
| Journal | American Journal of Physiology: Gastrointestinal and Liver Physiology |
| Volume | 284 |
| Issue number | 4 |
| Pages (from-to) | G670-82 |
| ISSN | 0193-1857 |
| DOIs | |
| Publication status | Published - Apr 2003 |
Keywords
- Adaptation, Physiological
- Animals
- Apoptosis
- Body Weight
- Cell Division
- Eating
- Enterocytes
- Glucagon
- Glucagon-Like Peptide 2
- Glucagon-Like Peptides
- Ileum
- Jejunum
- Male
- Parenteral Nutrition
- Peptides
- Proglucagon
- Protein Precursors
- Rats
- Rats, Sprague-Dawley
- Sucrase