Relative gene transcription and pathogenicity of enterohemorrhagic Escherichia coli after long-term adaptation to acid and salt stress

Inger Olesen; Lene Jespersen

doi:10.1016/j.ijfoodmicro.2010.05.019

Relative gene transcription and pathogenicity of enterohemorrhagic Escherichia coli after long-term adaptation to acid and salt stress

21 Citations (Scopus)

Abstract

Relative gene transcription and virulence potential, as measured by a Caco-2 adhesion assay, were investigated for three enterohemorrhagic Escherichia coli (EHEC) strains after long-term adaptation for 24 h to acid (BHI pH 5.5) and salt (BHI 4.5% (w/v) NaCl) stress. Five virulence genes (eae, lpfA, stx1A, stx2A and tir) and three stress response genes (asr, gadA and rpoS) were selected for gene transcription studies and compared to the relative transcription after growth at standard condition (BHI pH 7.0). In general, long-term adaptation to acid and salt stress significantly repressed the relative transcription of the virulence genes and induced relative transcription of the stress response genes though some strain variations were observed: the stress dependent gene transcription profiles obtained for STEC2 (O157:H7, minced beef) and c196-01 (O157:H-, clinical origin) were more similar compared to EDL933 (O157:H7, raw hamburger). Long-term adaptation to salt stress significantly increased the adhesion of all three EHEC strains to Caco-2 compared to the non-stressed controls. The present study shows that long-term adaptation to food related stress factors such as acid and salt is capable of changing the relative transcription of important virulence and stress response genes and increasing the virulence potential as measured by adhesion to the human colonic epithelial cell line, Caco-2.

Original language	English
Journal	International Journal of Food Microbiology
Volume	141
Issue number	3
Pages (from-to)	248-253
Number of pages	6
ISSN	0168-1605
DOIs	https://doi.org/10.1016/j.ijfoodmicro.2010.05.019
Publication status	Published - Jul 2010

Keywords

Former LIFE faculty

Access to Document

10.1016/j.ijfoodmicro.2010.05.019

Cite this

@article{0f12b9d5c9cf491185db5f313eff5624,

title = "Relative gene transcription and pathogenicity of enterohemorrhagic Escherichia coli after long-term adaptation to acid and salt stress",

abstract = "Relative gene transcription and virulence potential, as measured by a Caco-2 adhesion assay, were investigated for three enterohemorrhagic Escherichia coli (EHEC) strains after long-term adaptation for 24 h to acid (BHI pH 5.5) and salt (BHI 4.5% (w/v) NaCl) stress. Five virulence genes (eae, lpfA, stx1A, stx2A and tir) and three stress response genes (asr, gadA and rpoS) were selected for gene transcription studies and compared to the relative transcription after growth at standard condition (BHI pH 7.0). In general, long-term adaptation to acid and salt stress significantly repressed the relative transcription of the virulence genes and induced relative transcription of the stress response genes though some strain variations were observed: the stress dependent gene transcription profiles obtained for STEC2 (O157:H7, minced beef) and c196-01 (O157:H-, clinical origin) were more similar compared to EDL933 (O157:H7, raw hamburger). Long-term adaptation to salt stress significantly increased the adhesion of all three EHEC strains to Caco-2 compared to the non-stressed controls. The present study shows that long-term adaptation to food related stress factors such as acid and salt is capable of changing the relative transcription of important virulence and stress response genes and increasing the virulence potential as measured by adhesion to the human colonic epithelial cell line, Caco-2.",

keywords = "Former LIFE faculty, EHEC 0157:H7, Stress response, Virulence, Gene transcription, Caco-2",

author = "Inger Olesen and Lene Jespersen",

year = "2010",

month = jul,

doi = "10.1016/j.ijfoodmicro.2010.05.019",

language = "English",

volume = "141",

pages = "248--253",

journal = "International Journal of Food Microbiology",

issn = "0168-1605",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Relative gene transcription and pathogenicity of enterohemorrhagic Escherichia coli after long-term adaptation to acid and salt stress

AU - Olesen, Inger

AU - Jespersen, Lene

PY - 2010/7

Y1 - 2010/7

N2 - Relative gene transcription and virulence potential, as measured by a Caco-2 adhesion assay, were investigated for three enterohemorrhagic Escherichia coli (EHEC) strains after long-term adaptation for 24 h to acid (BHI pH 5.5) and salt (BHI 4.5% (w/v) NaCl) stress. Five virulence genes (eae, lpfA, stx1A, stx2A and tir) and three stress response genes (asr, gadA and rpoS) were selected for gene transcription studies and compared to the relative transcription after growth at standard condition (BHI pH 7.0). In general, long-term adaptation to acid and salt stress significantly repressed the relative transcription of the virulence genes and induced relative transcription of the stress response genes though some strain variations were observed: the stress dependent gene transcription profiles obtained for STEC2 (O157:H7, minced beef) and c196-01 (O157:H-, clinical origin) were more similar compared to EDL933 (O157:H7, raw hamburger). Long-term adaptation to salt stress significantly increased the adhesion of all three EHEC strains to Caco-2 compared to the non-stressed controls. The present study shows that long-term adaptation to food related stress factors such as acid and salt is capable of changing the relative transcription of important virulence and stress response genes and increasing the virulence potential as measured by adhesion to the human colonic epithelial cell line, Caco-2.

AB - Relative gene transcription and virulence potential, as measured by a Caco-2 adhesion assay, were investigated for three enterohemorrhagic Escherichia coli (EHEC) strains after long-term adaptation for 24 h to acid (BHI pH 5.5) and salt (BHI 4.5% (w/v) NaCl) stress. Five virulence genes (eae, lpfA, stx1A, stx2A and tir) and three stress response genes (asr, gadA and rpoS) were selected for gene transcription studies and compared to the relative transcription after growth at standard condition (BHI pH 7.0). In general, long-term adaptation to acid and salt stress significantly repressed the relative transcription of the virulence genes and induced relative transcription of the stress response genes though some strain variations were observed: the stress dependent gene transcription profiles obtained for STEC2 (O157:H7, minced beef) and c196-01 (O157:H-, clinical origin) were more similar compared to EDL933 (O157:H7, raw hamburger). Long-term adaptation to salt stress significantly increased the adhesion of all three EHEC strains to Caco-2 compared to the non-stressed controls. The present study shows that long-term adaptation to food related stress factors such as acid and salt is capable of changing the relative transcription of important virulence and stress response genes and increasing the virulence potential as measured by adhesion to the human colonic epithelial cell line, Caco-2.

KW - Former LIFE faculty

KW - EHEC 0157:H7

KW - Stress response

KW - Virulence

KW - Gene transcription

KW - Caco-2

U2 - 10.1016/j.ijfoodmicro.2010.05.019

DO - 10.1016/j.ijfoodmicro.2010.05.019

M3 - Journal article

SN - 0168-1605

VL - 141

SP - 248

EP - 253

JO - International Journal of Food Microbiology

JF - International Journal of Food Microbiology

IS - 3

ER -