Recovery of valuable peptides from marine protein hydrolysate by electrodialysis with ultrafiltration membrane: impact of ionic strength

Shyam Suwal; Cyril Roblet; Jean Amiot; Alain Doyen; Lucie Beaulieu; Jean Legault; Laurent Bazinet

doi:10.1016/j.foodres.2014.06.031

Recovery of valuable peptides from marine protein hydrolysate by electrodialysis with ultrafiltration membrane: impact of ionic strength

Shyam Suwal, Cyril Roblet, Jean Amiot, Alain Doyen, Lucie Beaulieu, Jean Legault, Laurent Bazinet

27 Citations (Scopus)

Abstract

Electrodialysis with ultrafiltration membrane (EDUF) has been used to selectively separate and concentrate peptides from protein hydrolysates. Therefore, comprehension and optimization of electrodialytic parameters involved in electrodialysis with ultrafiltration membrane (EDUF) are crucial for its industrial application. The aim of the present work was to study the effect of KCl concentrations (1, 3 and 5g/L) in recovery compartment on local electric field (LEF), total charge transport, energy consumption, peptide migration rate and selectivity during EDUF of snow crab by product hydrolysate (SCBH). The highest peptide migration rate (13.76±3.64g/m2.h) with the lowest relative energy consumption (132.85±15.67W.h/g) was recovered for the first time to our best knowledge in EDUF process whatsoever the KCl conditions. Furthermore, constant LEF and linear peptide migration rate were obtained for all the conditions tested by regulating the conductivities to their initial values during the EDUF process. More than 85% of total peptides in KCl fractions had molecular sizes that ranged from 300 to 600Da. In addition, KCl fractions were enriched with cationic peptides containing arginine and lysine and/or free arginine (Arg) and lysine (Lys). Furthermore, the relative abundance of Arg increased by 48%, 99% and 114% and that of Lys by 87%, 150% and 180% respectively in 1, 3 and 5g/L KCl concentrations in comparison to SCBH. An increase in ionic strength amplified the negative charge density of UF membrane. This increase in negative charge density would induce (1) an increase in effective pore size due to the electrostatic repulsion between ions and (2) a thinner hydration layer due to salting out effect at pore walls.

Original language	English
Journal	Food Research International
Volume	65
Issue number	PC
Pages (from-to)	407-415
Number of pages	9
ISSN	0963-9969
DOIs	https://doi.org/10.1016/j.foodres.2014.06.031
Publication status	Published - 1 Nov 2014

Keywords

KCl concentration on EDUF efficiency
Peptide migration rate
Peptide selective separation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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http://www.mendeley.com/research/recovery-valuable-peptides-marine-protein-hydrolysate-electrodialysis-ultrafiltration-membrane-impac

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@article{e0752fc01bad48ab9159bced0d1819d3,

title = "Recovery of valuable peptides from marine protein hydrolysate by electrodialysis with ultrafiltration membrane: impact of ionic strength",

abstract = "Electrodialysis with ultrafiltration membrane (EDUF) has been used to selectively separate and concentrate peptides from protein hydrolysates. Therefore, comprehension and optimization of electrodialytic parameters involved in electrodialysis with ultrafiltration membrane (EDUF) are crucial for its industrial application. The aim of the present work was to study the effect of KCl concentrations (1, 3 and 5g/L) in recovery compartment on local electric field (LEF), total charge transport, energy consumption, peptide migration rate and selectivity during EDUF of snow crab by product hydrolysate (SCBH). The highest peptide migration rate (13.76±3.64g/m2.h) with the lowest relative energy consumption (132.85±15.67W.h/g) was recovered for the first time to our best knowledge in EDUF process whatsoever the KCl conditions. Furthermore, constant LEF and linear peptide migration rate were obtained for all the conditions tested by regulating the conductivities to their initial values during the EDUF process. More than 85% of total peptides in KCl fractions had molecular sizes that ranged from 300 to 600Da. In addition, KCl fractions were enriched with cationic peptides containing arginine and lysine and/or free arginine (Arg) and lysine (Lys). Furthermore, the relative abundance of Arg increased by 48%, 99% and 114% and that of Lys by 87%, 150% and 180% respectively in 1, 3 and 5g/L KCl concentrations in comparison to SCBH. An increase in ionic strength amplified the negative charge density of UF membrane. This increase in negative charge density would induce (1) an increase in effective pore size due to the electrostatic repulsion between ions and (2) a thinner hydration layer due to salting out effect at pore walls.",

keywords = "KCl concentration on EDUF efficiency, Peptide migration rate, Peptide selective separation",

author = "Shyam Suwal and Cyril Roblet and Jean Amiot and Alain Doyen and Lucie Beaulieu and Jean Legault and Laurent Bazinet",

year = "2014",

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doi = "10.1016/j.foodres.2014.06.031",

language = "English",

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T1 - Recovery of valuable peptides from marine protein hydrolysate by electrodialysis with ultrafiltration membrane: impact of ionic strength

AU - Suwal, Shyam

AU - Roblet, Cyril

AU - Amiot, Jean

AU - Doyen, Alain

AU - Beaulieu, Lucie

AU - Legault, Jean

AU - Bazinet, Laurent

PY - 2014/11/1

Y1 - 2014/11/1

N2 - Electrodialysis with ultrafiltration membrane (EDUF) has been used to selectively separate and concentrate peptides from protein hydrolysates. Therefore, comprehension and optimization of electrodialytic parameters involved in electrodialysis with ultrafiltration membrane (EDUF) are crucial for its industrial application. The aim of the present work was to study the effect of KCl concentrations (1, 3 and 5g/L) in recovery compartment on local electric field (LEF), total charge transport, energy consumption, peptide migration rate and selectivity during EDUF of snow crab by product hydrolysate (SCBH). The highest peptide migration rate (13.76±3.64g/m2.h) with the lowest relative energy consumption (132.85±15.67W.h/g) was recovered for the first time to our best knowledge in EDUF process whatsoever the KCl conditions. Furthermore, constant LEF and linear peptide migration rate were obtained for all the conditions tested by regulating the conductivities to their initial values during the EDUF process. More than 85% of total peptides in KCl fractions had molecular sizes that ranged from 300 to 600Da. In addition, KCl fractions were enriched with cationic peptides containing arginine and lysine and/or free arginine (Arg) and lysine (Lys). Furthermore, the relative abundance of Arg increased by 48%, 99% and 114% and that of Lys by 87%, 150% and 180% respectively in 1, 3 and 5g/L KCl concentrations in comparison to SCBH. An increase in ionic strength amplified the negative charge density of UF membrane. This increase in negative charge density would induce (1) an increase in effective pore size due to the electrostatic repulsion between ions and (2) a thinner hydration layer due to salting out effect at pore walls.

AB - Electrodialysis with ultrafiltration membrane (EDUF) has been used to selectively separate and concentrate peptides from protein hydrolysates. Therefore, comprehension and optimization of electrodialytic parameters involved in electrodialysis with ultrafiltration membrane (EDUF) are crucial for its industrial application. The aim of the present work was to study the effect of KCl concentrations (1, 3 and 5g/L) in recovery compartment on local electric field (LEF), total charge transport, energy consumption, peptide migration rate and selectivity during EDUF of snow crab by product hydrolysate (SCBH). The highest peptide migration rate (13.76±3.64g/m2.h) with the lowest relative energy consumption (132.85±15.67W.h/g) was recovered for the first time to our best knowledge in EDUF process whatsoever the KCl conditions. Furthermore, constant LEF and linear peptide migration rate were obtained for all the conditions tested by regulating the conductivities to their initial values during the EDUF process. More than 85% of total peptides in KCl fractions had molecular sizes that ranged from 300 to 600Da. In addition, KCl fractions were enriched with cationic peptides containing arginine and lysine and/or free arginine (Arg) and lysine (Lys). Furthermore, the relative abundance of Arg increased by 48%, 99% and 114% and that of Lys by 87%, 150% and 180% respectively in 1, 3 and 5g/L KCl concentrations in comparison to SCBH. An increase in ionic strength amplified the negative charge density of UF membrane. This increase in negative charge density would induce (1) an increase in effective pore size due to the electrostatic repulsion between ions and (2) a thinner hydration layer due to salting out effect at pore walls.

KW - KCl concentration on EDUF efficiency

KW - Peptide migration rate

KW - Peptide selective separation

U2 - 10.1016/j.foodres.2014.06.031

DO - 10.1016/j.foodres.2014.06.031

M3 - Journal article

SN - 0963-9969

VL - 65

SP - 407

EP - 415

JO - Food Research International

JF - Food Research International

IS - PC

ER -

Recovery of valuable peptides from marine protein hydrolysate by electrodialysis with ultrafiltration membrane: impact of ionic strength

Abstract

Keywords

UN SDGs

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